Langrehr J M, Hoffman R A, Banner B, Stangl M J, Monyhan H, Lee K K, Schraut W H
Department of Surgery, School of Medicine, University of Pittsburgh, Pennsylvania 15261.
Transplantation. 1991 Sep;52(3):399-405. doi: 10.1097/00007890-199109000-00002.
This study was undertaken to investigate under which circumstances graft versus host disease occurs following fully allogenic small bowel transplantation in the rat. To facilitate the development of GVHD, Brown-Norway donors were specifically sensitized against the Lewis hosts prior to transplantation. Additionally, the Lewis recipients were immunocompromised before transplantation using splenectomy, cyclosporine, and antilymphocyte serum. No further immunosuppressive therapy was administered after transplantation. When all pretreatment regimens were used, acute lethal GVHD arose in two of nine animals (22%), whereas in two animals (22%) signs of acute GVHD and rejection were observed concurrently. When recipients of sensitized grafts were pretreated with CsA alone, one of eight animals (12.5%) showed signs of GVHD and rejection. All other animals died of acute rejection without clinical signs of acute GVHD. However, histological signs of GVHD were observed frequently in hosts grafted with a sensitized small bowel transplant. These data show that acute lethal GVHD can occur when an immunocompromised host is grafted with a sensitized intestinal transplant.
本研究旨在调查大鼠全异体小肠移植后在何种情况下会发生移植物抗宿主病。为促进移植物抗宿主病的发展，在移植前，将布朗 - 挪威供体特意致敏以对抗刘易斯宿主。此外，在移植前，通过脾切除术、环孢素和抗淋巴细胞血清使刘易斯受体免疫功能受损。移植后未再给予进一步的免疫抑制治疗。当使用所有预处理方案时，9只动物中有2只（22%）出现急性致死性移植物抗宿主病，而在2只动物（22%）中同时观察到急性移植物抗宿主病和排斥反应的迹象。当仅用环孢素预处理致敏移植物的受体时，8只动物中有1只（12.5%）出现移植物抗宿主病和排斥反应的迹象。所有其他动物死于急性排斥反应，无急性移植物抗宿主病的临床症状。然而，在接受致敏小肠移植的宿主中经常观察到移植物抗宿主病的组织学迹象。这些数据表明，当免疫功能受损的宿主接受致敏肠道移植时，可能会发生急性致死性移植物抗宿主病。
