Pirenne J, Williams J G, Mayoral J L, Nakhleh R E, Dunn D L
Department of Surgery, University of Minnesota, Minneapolis 55455.
Transplantation. 1991 Jan;51(1):51-7. doi: 10.1097/00007890-199101000-00007.
Transplantation of a Lewis rat small bowel allograft (SBTx) into Lewis x Brown Norway F1 (LBNF1) hybrid recipients provokes lethal graft-versus-host disease. GVHD in F1 hybrid rats inoculated with large number of parental lymphocytes may be abrogated by prior treatment of F1 hybrids with a sublethal dose (SLD) of the same parental lymphocytes. Therefore, we sought to determine whether this type of immunomodulation would prevent GVHD after SBTx. We examined whether pretreatment of LBNF1 recipients with a SLD of parental lymphocytes, or with a revascularized segment of parental small bowel, might ameliorate GVHD following transplantation of the entire parental small bowel. Nonpretreated LBNF1 recipients died of GVHD after entire Lew SBTx; 95% of LBNF1 recipients pretreated with SLD of Lew lymphocytes and 84% of LBNF1 recipients first transplanted with a segment of Lew small bowel survived GVHD induced by entire Lew SBTx 10 days later. Of LBNF1 recipients pretreated with SLD of Brown Norway lymphocytes or first transplanted with a segment of BN small bowel, none were protected from GVHD induced by entire Lew SBTx 10 days later. We concluded that pretreatment of LBNF1 recipients either with an SLD of parental lymphocytes or with a segment of parental small bowel provides profound protection from the effects of GVHD following transplantation of an entire small bowel of the same parental strain specificity.
将Lewis大鼠小肠同种异体移植（SBTx）到Lewis×Brown Norway F1（LBNF1）杂交受体中会引发致命的移植物抗宿主病。用大量亲代淋巴细胞接种的F1杂交大鼠中的移植物抗宿主病，可通过先用亚致死剂量（SLD）的相同亲代淋巴细胞对F1杂交大鼠进行预处理来消除。因此，我们试图确定这种免疫调节类型是否能预防SBTx后的移植物抗宿主病。我们研究了用SLD的亲代淋巴细胞或用亲代小肠的血管化节段对LBNF1受体进行预处理，是否能改善整个亲代小肠移植后的移植物抗宿主病。未进行预处理的LBNF1受体在整个Lewis SBTx后死于移植物抗宿主病；用SLD的Lewis淋巴细胞预处理的LBNF1受体中有95%以及首先移植一段Lewis小肠的LBNF1受体中有84%在10天后存活下来，未死于由整个Lewis SBTx诱导的移植物抗宿主病。在用SLD的Brown Norway淋巴细胞预处理或首先移植一段BN小肠的LBNF1受体中，10天后没有一只能够免受由整个Lewis SBTx诱导的移植物抗宿主病的影响。我们得出结论，用SLD的亲代淋巴细胞或一段亲代小肠对LBNF1受体进行预处理，可在移植相同亲代品系特异性的整个小肠后，为其提供对移植物抗宿主病影响的深度保护。
