Antonelli Marta Letizia, Faberi Angelo, Pastorini Elisabetta, Samperi Roberto, Laganà Aldo
Dipartimento di Chimica, Università "La Sapienza", Piazzale Aldo Moro 5, 00185 Roma, Italy.
Talanta. 2005 May 15;66(4):1025-33. doi: 10.1016/j.talanta.2005.01.004.
Phytoestrogens are diphenolic compounds that are present in several edible plants and are particularly abundant in soybeans. Because of their estrogenical, antriestrogenical, anticarcinogenic and antioxidant activities in animal and humans, they became of great interest. Dietary factors are considered important in determination of risks, in fact, studies have revealed beneficial or protective effects of the consumption of vegetables, in particular soy and soybean products. So that in the present paper the simultaneous determination of eight isoflavones and coumestrol in vegetables is reported. The quantitative analysis has been made by means of LC separation combined with tandem mass spectrometry. In particular, a new simple and fast extraction methodology and a clean-up, based on cold aided de-fatting, is proposed. Method performance was evaluated by comparison with a reference procedure. The developed procedure was then used for a survey of phytoestrogens concentration in some selected vegetables.
植物雌激素是存在于多种可食用植物中的双酚类化合物，在大豆中含量尤为丰富。由于它们在动物和人体中具有雌激素、抗雌激素、抗癌和抗氧化活性，因此备受关注。饮食因素在风险判定中被认为很重要，事实上，研究已经揭示了食用蔬菜，特别是大豆及大豆制品的有益或保护作用。因此，本文报道了同时测定蔬菜中八种异黄酮和香豆雌酚的方法。定量分析采用液相色谱分离结合串联质谱法进行。特别是，提出了一种基于冷辅助脱脂的新的简单快速提取方法和净化方法。通过与参考方法比较评估了方法性能。然后将所开发的方法用于一些选定蔬菜中植物雌激素浓度的调查。
