Peuravuori Juhani, Lehtonen Tero, Lepane Viia, Pihlaja Kalevi
Department of Chemistry, Physical Chemistry, University of Turku, FIN-20014 Turku, Finland.
Talanta. 2005 Jul 15;67(1):103-11. doi: 10.1016/j.talanta.2005.02.011. Epub 2005 Mar 17.
Capillary zone electrophoresis (CZE) with UV detection (254nm) was applied to characterize aquatic dissolved humic matter (DHM) from different environmental sources (lake, river and sea waters, in all 15 different samples). A series of separation examples of DHMs using a polyvinyl alcohol (PVA)-coated silica open tubular capillary were carried out in a phosphate buffer (40mM) as a background electrolyte at neutral acidity (pH 6.8). The separative power of electropherograms was reasonable and the reproducibility was above the mark. Each electropherogram was characteristic of the corresponding humic sample. Special functional fulvic and humic acids or their overall mixtures separated with XAD, DAX and DEAE sorbing solids as well as the original dissolved organic matter (DOM) were nicely differentiated according to their environmental sources. The PVA coating of open tubular silica capillaries seems to be very potential in electrophoretic characterization and separation of different humic solutes at neutral acidities with low sample concentrations thus permitting a workable technique, in a growing series of CZE studies, for better compared results from different studies.
采用紫外检测（254nm）的毛细管区带电泳（CZE）对来自不同环境来源（湖泊、河流和海水，共15个不同样本）的水生溶解腐殖质（DHM）进行表征。在以磷酸盐缓冲液（40mM）作为背景电解质、中性酸度（pH 6.8）的条件下，使用聚乙烯醇（PVA）涂层的硅胶开口管毛细管对一系列DHM进行分离。电泳图谱的分离能力合理，重现性良好。每个电泳图谱都是相应腐殖质样品的特征。用XAD、DAX和DEAE吸附剂分离得到的特殊功能性富里酸和腐殖酸或其整体混合物以及原始溶解有机物（DOM），根据其环境来源得到了很好的区分。在低样品浓度的中性酸度条件下，开口管硅胶毛细管的PVA涂层在不同腐殖质溶质的电泳表征和分离方面似乎非常有潜力，从而在越来越多的CZE研究中允许采用一种可行的技术，以获得不同研究之间更好的可比结果。
