Burger Fiona, Dawson Michael, Roux Claude, Maynard Philip, Doble Philip, Kirkbride Paul
Centre for Forensic Science, University of Technology Sydney, Australia.
Talanta. 2005 Aug 15;67(2):368-76. doi: 10.1016/j.talanta.2005.03.040. Epub 2005 Jul 12.
Condoms may offer sexual assailants a simple and relatively effective means by which they may remove and dispose of the biological evidence of their contact with the victim. Without this valuable probative evidence, the investigator may need to turn to such alternative forms of physical evidence, such as the residue remaining from the condom lubricant, and any other additional lubricating substances, which may have been used by the assailant. In this study, 68 different condom and personal lubricant samples were analysed using micellar electrokinetic capillary chromatography (MEKC) with ultraviolet absorbance detection. The electropherograms were processed by principal component analysis (PCA) and classified with linear discriminant analysis. This classified 233 samples out of 263 into their correct sample group. An alternative method of classification in which the sample electropherograms were converted to vectors and a correlation was determined by the cosine of the angles between these vectors. This correctly classified 172 samples of the 296. Results indicate that the combination of CE with chemometric data treatment offers the potential of being a rapid and efficient means by which condom and personal lubricant samples may be differentiated. Although this analytical method at present lacks the sensitivity required for sexual assault swab analysis, it is hoped that future developments in instrumentation detection capabilities will permit its use one day as a routine casework tool.
避孕套可能为性侵犯者提供一种简单且相对有效的手段，使其能够清除并处理与受害者接触的生物证据。没有了这一宝贵的证据，调查人员可能需要转而寻找其他形式的物证，比如避孕套润滑剂残留以及袭击者可能使用过的任何其他额外润滑物质。在本研究中，使用胶束电动毛细管色谱法（MEKC）结合紫外吸收检测对68种不同的避孕套和个人润滑剂样本进行了分析。通过主成分分析（PCA）处理电泳图，并采用线性判别分析进行分类。在263个样本中，有233个被正确分类到其所属样本组。另一种分类方法是将样本电泳图转换为向量，并通过这些向量之间夹角的余弦来确定相关性。在296个样本中，有172个被正确分类。结果表明，毛细管电泳与化学计量学数据处理相结合，有可能成为一种快速有效的方法来区分避孕套和个人润滑剂样本。尽管这种分析方法目前缺乏性侵犯拭子分析所需的灵敏度，但希望仪器检测能力的未来发展有朝一日能使其作为常规办案工具得以使用。
