Ma Guangyu, Cheng Quan
Department of Chemistry, University of California, Riverside, CA 92521, USA.
Talanta. 2005 Sep 15;67(3):514-9. doi: 10.1016/j.talanta.2005.06.032. Epub 2005 Aug 2.
We report a nanoscale lipid membrane-based sensor of conjugated polydiacetylene (PDA) vesicles for fluorescence detection of organic amines. The vesicle sensor was constructed by incorporation of a BODIPY fluorescent dye into the PDA vesicles. The fluorescent properties of the resulting vesicles can be manipulated by adjusting lipid components, and are controlled by environmental and solution conditions. The fluorescence of the BODIPY dye was significantly quenched in the polymerization of diacetylene lipid vesicles by a UV irradiation process. However, it was sufficiently recovered by external stimuli such as a hike of solution pH. The fluorescence recovery process was reversible, and a decrease in solution pH resulted in repeated quenching. The reported system transforms an external stimulus into a large fluorescence intensity change, demonstrating great potential in developing new signal reporting method for biosensor design. The quench-recovery phenomenon of the BODIPY-PDA is believed to be related to the energy transfer between the dye and the PDA conjugate backbone. The vesicle sensor was applied for detecting an organic amine, triethylamine (TEA) and a large linear relationship was obtained between the increase in fluorescence intensity and the concentrations of TEA. The detection limit of TEA by vesicle sensors using fluorescence recovery was found to be 10muM.
我们报道了一种基于纳米级脂质膜的共轭聚二乙炔（PDA）囊泡传感器，用于有机胺的荧光检测。该囊泡传感器是通过将一种BODIPY荧光染料掺入PDA囊泡中构建而成。所得囊泡的荧光特性可通过调节脂质成分来操控，并受环境和溶液条件的控制。在紫外光照射过程中，二乙炔脂质囊泡聚合时，BODIPY染料的荧光会显著猝灭。然而，通过诸如提高溶液pH值等外部刺激，其荧光能充分恢复。荧光恢复过程是可逆的，溶液pH值降低会导致荧光再次猝灭。所报道的系统将外部刺激转化为较大的荧光强度变化，这表明在开发用于生物传感器设计的新信号报告方法方面具有巨大潜力。BODIPY-PDA的猝灭-恢复现象被认为与染料和PDA共轭主链之间的能量转移有关。该囊泡传感器用于检测有机胺三乙胺（TEA），荧光强度的增加与TEA浓度之间呈现出较大的线性关系。利用荧光恢复的囊泡传感器对TEA的检测限为10μM。
