Guo Huishi, He Nongyue, Ge Shuxun, Yang Di, Zhang Jinan
State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, China; Institute of Cardiovascular Disease, Nanjing Medical University, Nanjing 210029, China.
Talanta. 2005 Nov 15;68(1):61-6. doi: 10.1016/j.talanta.2005.04.067. Epub 2005 Jun 8.
An anodic stripping voltammetric method for the determination of cardiac troponin I (cTnI) at a MCM-41 mesoporous material modified carbon paste electrode (MCM-MCPE) was investigated. The test was based on the dual monoclonal antibody "sandwich" principle using colloidal gold as a labeled substrate. Four main steps were carried out to obtain the analytical signal, i.e. electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. The anodic stripping peak current increased linearly with the concentration of cTnI over the range of 0.8-5.0ng/ml. A detection limit of 0.5ng/ml was obtained. The established method was applied to detect cTnI in acute myocardial infarction (AMI) samples using routine enzyme-linked immunoadsorbent assay (ELISA) for comparison analysis, and good results were obtained.
研究了一种在MCM-41介孔材料修饰碳糊电极（MCM-MCPE）上测定心肌肌钙蛋白I（cTnI）的阳极溶出伏安法。该检测基于使用胶体金作为标记底物的双单克隆抗体“夹心”原理。通过四个主要步骤获得分析信号，即电极制备、免疫反应、银增强和阳极溶出伏安检测。在0.8 - 5.0 ng/ml范围内，阳极溶出峰电流与cTnI浓度呈线性增加。获得了0.5 ng/ml的检测限。将所建立的方法应用于急性心肌梗死（AMI）样本中cTnI的检测，并使用常规酶联免疫吸附测定（ELISA）进行比较分析，获得了良好的结果。
