Chumbimuni-Torres Karin Y, Dai Zong, Rubinova Nastassia, Xiang Yun, Pretsch Ernö, Wang Joseph, Bakker Eric
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
J Am Chem Soc. 2006 Oct 25;128(42):13676-7. doi: 10.1021/ja065899k.
We report here for the first time on the use of potentiometry for ultrasensitive nanoparticle-based detection of protein interactions. A silver ion-selective microelectrode is used to detect silver ions oxidatively released from silver enlarged gold nanoparticle labels in a sandwich immunoassay. Since potentiometry is expected to largely maintain its analytical characteristics upon reducing the sample volume, it is anticipated that this approach may form the basis for bioassays with attractive detection limits.
我们首次在此报告电位分析法用于基于超灵敏纳米颗粒的蛋白质相互作用检测。在夹心免疫分析中，使用银离子选择性微电极检测从银增强金纳米颗粒标记物中氧化释放的银离子。由于预计电位分析法在减小样品体积时能在很大程度上保持其分析特性，因此预计这种方法可能成为具有吸引人的检测限的生物分析的基础。
