使用阴离子交换柱-流动注射分析系统和析因设计研究荧光法测定葡萄酒和苹果酒中的组胺。

Fluorimetric determination of histamine in wine and cider by using an anion-exchange column-FIA system and factorial design study.

作者信息

Del Campo Gloria, Gallego Beatriz, Berregi Iñaki

机构信息

Applied Chemistry Department, Faculty of Chemistry, University of the Basque Country, Manuel Lardizabal 3, P.O. Box 1072, E-20018 San Sebastián, Spain.

出版信息

Talanta. 2006 Feb 15;68(4):1126-34. doi: 10.1016/j.talanta.2005.07.019. Epub 2005 Aug 19.

DOI:10.1016/j.talanta.2005.07.019

PMID:18970441

Abstract

A study has been performed of the conditions for the reaction of histamine with o-phthaldehyde in a flow injection analysis system employing three channels, using an anion-exchange column to eliminate sample matrix interferences. Factorial design was used to determine which operational parameters should be included in the optimization and their optimal values were found. The method developed shows good selectivity for histamine determination in alcoholic beverages. A linear response of up to 2.0mgl(-1) was observed and the detection and quantification limits were 30 and 101mugl(-1), respectively. The repeatability, measured by the R.S.D. for 10 replicate injections, was 0.84 and 0.52% for histamine solutions of 0.20 and 2.0mgl(-1), respectively. The recoveries obtained in wine and cider samples were close to 100% and a sample frequency of 24 samples per hour was achieved.

摘要

在采用三通道的流动注射分析系统中，对组胺与邻苯二甲醛反应的条件进行了研究，使用阴离子交换柱消除样品基质干扰。采用析因设计来确定优化过程中应包含哪些操作参数，并找到了它们的最佳值。所开发的方法对酒精饮料中组胺的测定具有良好的选择性。观察到线性响应高达2.0mg l⁻¹，检测限和定量限分别为30和101μg l⁻¹。通过10次重复进样的相对标准偏差（R.S.D.）衡量的重复性，对于0.20和2.0mg l⁻¹的组胺溶液分别为0.84%和0.52%。葡萄酒和苹果酒样品中的回收率接近100%，每小时可实现24个样品的进样频率。