Garcia Jerusa Simone, Magalhães Cristiana Schmidt de, Arruda Marco Aurélio Zezzi
Department of Analytical Chemistry, Institute of Chemistry, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
Talanta. 2006 Mar 15;69(1):1-15. doi: 10.1016/j.talanta.2005.08.041. Epub 2005 Oct 3.
This review describes recent tendencies for metal-binding and metalloprotein analysis, emphasizing metal quantification in proteins through X-ray, atomic absorption, mass spectrometric techniques, and others. Hyphenated techniques such as capillary electrophoresis-synchrotron radiation X-ray fluorescence (CE-SRXRF), laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), matrix-assisted laser desorption/ionisation-time-of-flight mass spectrometry (MALDI-TOF-MS), etc. are also presented. As protein separation techniques electrophoresis (mainly sodium dodecyl sulphate-polyacrylamide gel electrophoresis, SDS-PAGE), capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are indicated, due to their inherent sensitivity, resolution and/or easy implementation. Latest challenges in metallomics are also commented.
本综述描述了金属结合和金属蛋白分析的最新趋势，重点介绍了通过X射线、原子吸收、质谱技术等对蛋白质中的金属进行定量分析。还介绍了联用技术，如毛细管电泳-同步辐射X射线荧光光谱法（CE-SRXRF）、激光烧蚀-电感耦合等离子体质谱法（LA-ICP-MS）、基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF-MS）等。文中指出了蛋白质分离技术，如电泳（主要是十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，SDS-PAGE）、毛细管电泳（CE）和高效液相色谱（HPLC），因为它们具有固有的灵敏度、分辨率和/或易于实施的特点。文中还对金属组学的最新挑战进行了评论。
