采用蒸发光散射检测的高效液相色谱法同时分离九种不同类型的表面活性剂。
Simultaneous separation of nine surfactants of various types by HPLC with evaporative light scattering detection.
作者信息
Park Hong Soon, Ryu Ho Ryul, Rhee Choong Kyun
机构信息
Department of Chemistry, Chungnam National University, 220 Kung-dong, Deajeon 305-764, South Korea.
出版信息
Talanta. 2006 Oct 15;70(3):481-4. doi: 10.1016/j.talanta.2006.01.029. Epub 2006 Feb 28.
A simultaneous separation of cationic, amphoteric and nonioinc nine surfactants (DMDS, DMDP, DMDM, DMDL, BZC, CDE, A/O, SUNC, IMD) has been performed by a reverse phase-HPLC method utilizing a single J'sphere ODS (250mmx4.6mm, 4mum) column and a methanol-water containing 0.2% TFA eluent system within 60min. The observed precision in determination of concentration was less than 5% R.S.D., which revealed that ELSD was an effective tool to detect the various studied surfactants of low volatility without chromophore. In addition, the detection limits were in the concentration range of 3.5-10mug/mL, and the calibration curves, i.e. the log-log plots, were linear in the working range of 5-4600mug/mL with the slopes of 1.321-1.668. The application of the analytical procedure to three household products without pretreatment supported that the presented chromatographic method was simple to be practical for a routine analysis of commercial products.
采用反相高效液相色谱法，使用一根J'sphere ODS（250mm×4.6mm，4μm）色谱柱和含0.2%三氟乙酸的甲醇-水流动相体系，在60分钟内同时分离了阳离子、两性离子和非离子型九种表面活性剂（DMDS、DMDP、DMDM、DMDL、BZC、CDE、A/O、SUNC、IMD）。测定浓度时观察到的精密度相对标准偏差（R.S.D.）小于5%，这表明蒸发光散射检测器（ELSD）是检测各种低挥发性且无发色团的表面活性剂的有效工具。此外，检测限在3.5 - 10μg/mL浓度范围内，校准曲线（即对数-对数图）在5 - 4600μg/mL的工作范围内呈线性，斜率为1.321 - 1.668。该分析方法无需预处理直接应用于三种家用产品，表明所提出的色谱方法简单实用，可用于商业产品的常规分析。
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