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两种绵羊线虫寄生虫虫卵计数方法的准确性

Accuracy of two methods for counting eggs of sheep nematode parasites.

作者信息

Rossanigo C E, Gruner L

机构信息

INRA, Unité d'Ecologie des Parasites, Nouzilly, France.

出版信息

Vet Parasitol. 1991 Jul;39(1-2):115-21. doi: 10.1016/0304-4017(91)90067-6.

DOI:10.1016/0304-4017(91)90067-6
PMID:1897113
Abstract

A comparative study was carried out on eight species of sheep gastrointestinal nematodes in order to compare the value of two techniques for egg counting: the classical McMaster technique with saturated magnesium sulfate solution and a technique of egg extraction from faeces and counting by total collection after three centrifugations. Efficiency of extraction from 10 g of faeces was 95.9 to 99.5% according to the species of parasite, whereas the number of eggs counted by the McMaster technique represented only 16.5% of the total eggs present in the faeces. Advantages inherent in the use of these techniques are discussed.

摘要

对八种绵羊胃肠线虫进行了一项比较研究,以比较两种虫卵计数技术的价值:一种是使用饱和硫酸镁溶液的经典麦克马斯特技术,另一种是从粪便中提取虫卵并经过三次离心后通过全量收集进行计数的技术。根据寄生虫种类的不同,从10克粪便中提取虫卵的效率为95.9%至99.5%,而用麦克马斯特技术计数的虫卵数量仅占粪便中虫卵总数的16.5%。文中讨论了使用这些技术所固有的优点。

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