Bosco Antonio, Rinaldi Laura, Maurelli Maria P, Musella Vincenzo, Coles Gerald C, Cringoli Giuseppe
Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR Regione Campania, Naples, Italy.
Acta Parasitol. 2014 Oct;59(4):625-8. doi: 10.2478/s11686-014-0282-7. Epub 2014 Sep 20.
Three methods, FLOTAC, FECPAK and McMaster were compared for accuracy and sensitivity for counting numbers of nematode eggs in faeces of naturally infected cattle with high or low nematode egg counts. Only FLOTAC gave positive results for 12 replicates from pooled samples with low egg counts making it more sensitive than FECPAK (67%) and McMaster (41.7%). FLOTAC resulted in generally higher egg counts and lower coefficients of variation than the other two methods used. The reliability of FECPAK and McMaster is depended on the area under the slide counted. All three methods can be used for making decisions whether to treat but FLOTAC or Mini-FLOTAC should be used for faecal egg count reduction tests when lower egg counts are present.
比较了FLOTAC、FECPAK和麦克马斯特三种方法对自然感染线虫且线虫卵计数高或低的牛粪中线虫卵计数的准确性和敏感性。对于虫卵计数低的混合样本的12次重复检测，只有FLOTAC得出了阳性结果，这使其比FECPAK（67%）和麦克马斯特（41.7%）更敏感。与使用的其他两种方法相比，FLOTAC得出的虫卵计数通常更高，变异系数更低。FECPAK和麦克马斯特的可靠性取决于计数载玻片的面积。所有三种方法都可用于决定是否进行治疗，但当虫卵计数较低时，应使用FLOTAC或微型FLOTAC进行粪便虫卵计数减少试验。
