Imai S, Mukai Y, Takeda T, Abe Y, Nagano K, Kamada H, Nakagawa S, Tsunoda S, Tsutsumi Y
Laboratory of Pharmaceutical Proteomics, National Institute of Biomedical Innovation (NIBIO), Osaka, Japan.
Pharmazie. 2008 Oct;63(10):760-4.
The M13 phage display system is a powerful technology for engineering proteins such as functional mutant proteins and peptides. In this system, it is necessary that the protein is displayed on the phage surface. Therefore, its application is often limited when a protein is poorly displayed. In this study, we attempted to understand the relationship between a protein's properties and its display efficiency using the well-known pIII and pVIII type phage display system. The display of positively charged SV40 NLS and HIV-1 Tat peptides on pill was less efficient than that of the neutrally charged RGDS peptide. When different molecular weight proteins (1.5-58 kDa) were displayed on pIII and pVIII, their display efficiencies were directly influenced by their molecular weights. These results indicate the usefulness in predicting a desired protein's compatibility with protein and peptide engineering using the phage display system.
M13噬菌体展示系统是用于改造蛋白质（如功能性突变蛋白和肽）的一项强大技术。在该系统中，蛋白质必须展示在噬菌体表面。因此，当一种蛋白质展示效果不佳时，其应用往往会受到限制。在本研究中，我们试图利用著名的pIII和pVIII型噬菌体展示系统来了解蛋白质性质与其展示效率之间的关系。带正电荷的SV40核定位信号（NLS）和HIV-1反式激活因子（Tat）肽在pIII上的展示效率低于带中性电荷的RGDS肽。当不同分子量的蛋白质（1.5 - 58 kDa）展示在pIII和pVIII上时，它们的展示效率直接受到分子量的影响。这些结果表明，利用噬菌体展示系统预测所需蛋白质与蛋白质及肽工程的兼容性具有实用性。
