[福司可林对体外培养的小鼠T淋巴细胞增殖、细胞周期分布及活化的影响]
[Effects of Forskolin on proliferation cell-cycle distribution and activation of the murine T lymphocytes in vitro].
作者信息
Song Bing, Zeng Yao-Ying, Huang Xiu-Yan, Li Lin, Teng Fei, Yang Zhi, Yao Man-Lin
机构信息
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou 510632,China.
出版信息
Zhong Yao Cai. 2008 Jul;31(7):1008-12.
OBJECTIVE
To investigate the effects of Forskolin on activation, proliferation, and cell-cycle distribution of murine CD3+ T lymphocytes, and study the mechanisms of its immunosuppressive effect.
METHODS
Singel cell suspensions were prepared from murine lymph nodes. Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 activated by Con A, the proliferation index of activated mouse T lymphocytes was analyzed by CFDA-SE staining, the distribution of the cell cycle was analyzed by PI staining.
RESULTS
Forskolin (10(-7), 10(-6), 10(-5) M) could inhibit both the expression of CD69 on CD3+ T lymphocytes and T lymphocyte proliferation index stimulated by Con A in a dose-dependent manner. The C0/G1 of T lymphocytes increased but the S, G2/M phase decreased.
CONCLUSION
Forskolin can inhibit the activation and proliferation of murine T lymphocytes in vitro, and arrest activated T lymphocytes from G0/G1 to S or G2/M. Forskolin is a potential immunosupressive agent.
目的
研究福司可林对小鼠CD3+ T淋巴细胞活化、增殖及细胞周期分布的影响，并探讨其免疫抑制作用机制。
方法
从小鼠淋巴结制备单细胞悬液。采用荧光共轭单克隆抗体和流式细胞术检测Con A激活的CD69表达，用CFDA-SE染色分析活化小鼠T淋巴细胞的增殖指数，用PI染色分析细胞周期分布。
结果
福司可林（10(-7)、10(-6)、10(-5) M）能剂量依赖性抑制CD3+ T淋巴细胞上CD69的表达及Con A刺激的T淋巴细胞增殖指数。T淋巴细胞的G0/G1期增加，S期、G2/M期减少。
结论
福司可林可在体外抑制小鼠T淋巴细胞的活化和增殖，并使活化的T淋巴细胞停滞于G0/G1期，阻止其进入S期或G2/M期。福司可林是一种潜在的免疫抑制剂。
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