Liang Zhao-duan, Zeng Yao-ying, Huang Xiu-yan, He Fang
Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Apr;24(4):337-40.
To study the effect of Apigenin (AP) on the proliferation, cell cycle and apoptosis of mouse T cells in vitro.
The lymphocytes were prepared from lymph nodes and thymus of mice. The effect of AP on the proliferation of T in response to ConA at different concentrations (25, 50, 100, 150, 200 micromol/L) was detected by MTT. Cell cycle was measured by PI staining and FCM. The effect of Apigenin and Apigenin with DEX on T cell apoptosis was measured by Annexin V-FITC/PI double staining and FCM. The effect of different concentrations of AP cytotoxicity to T cells was measured by MTT.
25-200 micromol/L of AP didn't have cytotoxicity to T cells, but it had some inhibitory effect on T cells in response to ConA(P<0.01), arresting cell cycle at G0/G1 in a dose-dependent manner. Different concentrations of AP inhibited the apoptosis of T cells, especially those induced by DEX(P<0.01).
AP can inhibit the proliferation of mouse T cells in response to ConA, arrest cell cycle at G0/G1 and inhibit the apoptosis of T cells.
研究芹菜素（AP）对小鼠T细胞体外增殖、细胞周期及凋亡的影响。
从小鼠淋巴结和胸腺制备淋巴细胞。采用MTT法检测不同浓度（25、50、100、150、200 μmol/L）AP对T细胞在ConA刺激下增殖的影响。通过PI染色和流式细胞术检测细胞周期。采用Annexin V-FITC/PI双染和流式细胞术检测芹菜素及芹菜素与地塞米松联合作用对T细胞凋亡的影响。采用MTT法检测不同浓度AP对T细胞的细胞毒性作用。
25 - 200 μmol/L的AP对T细胞无细胞毒性，但对T细胞在ConA刺激下的增殖有一定抑制作用（P<0.01），并呈剂量依赖性地使细胞周期阻滞于G0/G1期。不同浓度的AP抑制T细胞凋亡，尤其是抑制地塞米松诱导的T细胞凋亡（P<0.01）。
AP可抑制小鼠T细胞在ConA刺激下的增殖，使细胞周期阻滞于G0/G1期，并抑制T细胞凋亡。
