芹菜素对小鼠T细胞体外增殖、细胞周期及凋亡的影响

[Effect of Apigenin on proliferation, cell cycle and apoptosis of mouse T cells in vitro].

作者信息

Liang Zhao-duan, Zeng Yao-ying, Huang Xiu-yan, He Fang

机构信息

Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Apr;24(4):337-40.

PMID:18394337

Abstract

AIM

To study the effect of Apigenin (AP) on the proliferation, cell cycle and apoptosis of mouse T cells in vitro.

METHODS

The lymphocytes were prepared from lymph nodes and thymus of mice. The effect of AP on the proliferation of T in response to ConA at different concentrations (25, 50, 100, 150, 200 micromol/L) was detected by MTT. Cell cycle was measured by PI staining and FCM. The effect of Apigenin and Apigenin with DEX on T cell apoptosis was measured by Annexin V-FITC/PI double staining and FCM. The effect of different concentrations of AP cytotoxicity to T cells was measured by MTT.

RESULTS

25-200 micromol/L of AP didn't have cytotoxicity to T cells, but it had some inhibitory effect on T cells in response to ConA(P<0.01), arresting cell cycle at G0/G1 in a dose-dependent manner. Different concentrations of AP inhibited the apoptosis of T cells, especially those induced by DEX(P<0.01).

CONCLUSION

AP can inhibit the proliferation of mouse T cells in response to ConA, arrest cell cycle at G0/G1 and inhibit the apoptosis of T cells.

摘要

目的

研究芹菜素（AP）对小鼠T细胞体外增殖、细胞周期及凋亡的影响。

方法

从小鼠淋巴结和胸腺制备淋巴细胞。采用MTT法检测不同浓度（25、50、100、150、200 μmol/L）AP对T细胞在ConA刺激下增殖的影响。通过PI染色和流式细胞术检测细胞周期。采用Annexin V-FITC/PI双染和流式细胞术检测芹菜素及芹菜素与地塞米松联合作用对T细胞凋亡的影响。采用MTT法检测不同浓度AP对T细胞的细胞毒性作用。