Kou Yi-Ying, Zha Xi-Duojie, Li Yong-Fang, Ge Ri-Li
Medical College Qinghai University, Xining 810001, China.
Zhong Yao Cai. 2008 Jul;31(7):1013-5.
To study the effect of Sanwei Tanxiang powder on myocardial pathologic change, myocardium lipid peroxidation and antioxidation on anesthetized rats' hearts against myocardial ischemia and reperfusion injury.
A model of regional myocardial ischemia-reperfusion injury (IR) was established by 30 min ligation of the left anterior descending coronary artery followed by 40 min reperfusion in hearts of anesthetized rats'. The experimental animals were randomly divided into the sham operation, IR model group, positive control group and two dose Sanwei Tanxiang powder groups. The changes of ECG, LDH, CK, SOD, GSH-Px and MDA in the heart were measured and ultrastructural pathological changes were observed.
In Sanwei Tanxiang groups the displacement of ST segment reduced (P < 0.05), the activity of LDH, CK decreased (P < 0.05), the activities of SOD, GSH-Px significantly increased and the contents of MDA reduced (P < 0.05). Moreover, the development of ultrastructral pathological changes obviously improved compared with the IR model group.
The protective effects of Sanwei Tanxiang powder on anesthetized rats' hearts against myocardial ischemia and reperfusion injury may be related to protecting the antioxidants and inhibiting the lipid peroxidation in the myocardium.
研究三味檀香散对麻醉大鼠心脏抗心肌缺血再灌注损伤时心肌病理变化、心肌脂质过氧化及抗氧化作用的影响。
通过结扎麻醉大鼠心脏左前降支30分钟，再灌注40分钟，建立局部心肌缺血再灌注损伤（IR）模型。将实验动物随机分为假手术组、IR模型组、阳性对照组和两个剂量的三味檀香散组。检测心脏中心电图、乳酸脱氢酶（LDH）、肌酸激酶（CK）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的变化，并观察超微结构病理变化。
三味檀香散组ST段位移减小（P<0.05），LDH、CK活性降低（P<0.05），SOD、GSH-Px活性显著升高，MDA含量降低（P<0.05）。此外，与IR模型组相比，超微结构病理变化的发展明显改善。
三味檀香散对麻醉大鼠心脏抗心肌缺血再灌注损伤的保护作用可能与保护抗氧化剂和抑制心肌脂质过氧化有关。
