Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, 825 NE 13(th) Street, Oklahoma City, OK 73104, USA.
Facultad de Ciencias Químicas, Dpto. Química Biológica Ranwel Caputto-CIQUIBIC, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Ciudad Universitaria de Córdoba, Córdoba X5000HUA, Argentina.
Curr Biol. 2020 Apr 6;30(7):1207-1216.e4. doi: 10.1016/j.cub.2020.01.054. Epub 2020 Feb 13.
Telomere-led rapid chromosome movements or rapid prophase movements direct fundamental meiotic processes required for successful haploidization of the genome. Critical components of the machinery that generates rapid prophase movements are unknown, and the mechanism underlying rapid prophase movements remains poorly understood. We identified S. cerevisiae Mps2 as the outer nuclear membrane protein that connects the LINC complex with the cytoskeleton. We also demonstrate that the motor Myo2 works together with Mps2 to couple the telomeres to the actin cytoskeleton. Further, we show that Csm4 interacts with Mps2 and is required for perinuclear localization of Myo2, implicating Csm4 as a regulator of the Mps2-Myo2 interaction. We propose a model in which the newly identified functions of Mps2 and Myo2 cooperate with Csm4 to drive chromosome movements in meiotic prophase by coupling telomeres to the actin cytoskeleton.
端粒驱动的快速染色体运动或快速前期运动指导着成功进行基因组单倍体化所需的基本减数分裂过程。产生快速前期运动的机制的关键组件尚不清楚，快速前期运动的机制仍知之甚少。我们鉴定出酿酒酵母 Mps2 是将 LINC 复合物与细胞骨架连接起来的核外膜蛋白。我们还证明，马达蛋白 Myo2 与 Mps2 一起作用，将端粒与肌动蛋白细胞骨架连接起来。此外，我们还表明 Csm4 与 Mps2 相互作用，并且对于 Myo2 的核周定位是必需的，这表明 Csm4 是 Mps2-Myo2 相互作用的调节剂。我们提出了一个模型，其中新鉴定出的 Mps2 和 Myo2 的功能与 Csm4 合作，通过将端粒与肌动蛋白细胞骨架连接起来，在减数分裂前期驱动染色体运动。
