Schäfer Stephan C, Pfnür Maria, Yerly Stephane, Fandel Thomas M, Jichlinski Patrice, Lehr Hans-Anton
University Institute of Pathology, CHUV, University of Lausanne, Lausanne, Switzerland.
Prostate. 2009 Feb 1;69(2):191-7. doi: 10.1002/pros.20868.
Most molecular techniques currently require fresh frozen tumor tissue, which in the case of prostatectomy specimen is a challenge to obtain for a variety of intrinsic reasons. Prostate cancers are usually located in the organ periphery and hence meticulous attention has to be paid to the relation between the tumor and the surgical margin. In this article we describe a new technique that allows to obtain fresh frozen tumor material in rather large quantities and without jeopardizing diagnostic accuracy.
An inner triangle, representing roughly 50% of the entire prostate tissue, is removed from native prostatectomy specimen and cryopreserved, leaving the periphery of the organ for routine histomorphological analysis. We have validated the technique using a series of 180 archived radical prostatectomy specimen that had been studied by histology in their entirety, as well as 42 prostatectomy specimen worked-up by the new technique.
The described technique is effective, yielding frozen tumor tissue in 84.2% of unilateral (<or=pT2b) and in 91.8% of bilateral (pT2c) carcinomas. The number of tumor containing tissue blocks ranged from 1 to 7 blocks per carcinoma (mean 5.6 +/- 3.2). The remaining peripheral tissue portion subjected to histological analysis allowed to estimate total tumor volume with a high degree to confidentiality (r(2) >or= 0.90 by linear regression analysis). The number of blocks to be routinely examined for each prostatectomy specimen is significantly reduced, thus saving costs without loss of diagnostic accuracy.
The described technique for prostate cancer cryoconservtion is feasible, highly standardized, effective, safe, and economic.
目前大多数分子技术需要新鲜冷冻的肿瘤组织，对于前列腺切除标本而言，由于各种内在原因，获取此类组织颇具挑战性。前列腺癌通常位于器官外周，因此必须密切关注肿瘤与手术切缘之间的关系。在本文中，我们描述了一种新技术，该技术能够获取大量新鲜冷冻的肿瘤材料，且不会影响诊断准确性。
从前列腺切除标本中切除一个大致占整个前列腺组织50%的内部三角形区域，并进行冷冻保存，将器官外周部分留作常规组织形态学分析。我们使用了一系列180例已进行完整组织学研究的存档根治性前列腺切除标本以及42例采用新技术处理的前列腺切除标本对该技术进行了验证。
所描述的技术是有效的，在单侧（≤pT2b）癌中，84.2%可获得冷冻肿瘤组织，在双侧（pT2c）癌中，91.8%可获得冷冻肿瘤组织。每个癌组织中含肿瘤的组织块数量为1至7块（平均5.6±3.2）。其余用于组织学分析的外周组织部分能够高度准确地估计肿瘤总体积（线性回归分析r²≥0.90）。每个前列腺切除标本常规检查的组织块数量显著减少，从而在不损失诊断准确性的情况下节省了成本。
所描述的前列腺癌冷冻保存技术是可行的、高度标准化的、有效的、安全的且经济的。
