Vlajnic Tatjana, Oeggerli Martin, Rentsch Cyrill, Püschel Heike, Zellweger Tobias, Thalmann George N, Ruiz Christian, Bubendorf Lukas
Institute of Pathology, University Hospital Basel, Schoenbeinstrasse 40, 4031, Basel, Switzerland,
Virchows Arch. 2014 Dec;465(6):637-42. doi: 10.1007/s00428-014-1665-8. Epub 2014 Oct 17.
Procurement of fresh tissue of prostate cancer is critical for biobanking and generation of xenograft models as an important preclinical step towards new therapeutic strategies in advanced prostate cancer. However, handling of fresh radical prostatectomy specimens has been notoriously challenging given the distinctive physical properties of prostate tissue and the difficulty to identify cancer foci on gross examination. Here, we have developed a novel approach using ceramic foam plates for processing freshly cut whole mount sections from radical prostatectomy specimens without compromising further diagnostic assessment. Forty-nine radical prostatectomy specimens were processed and sectioned from the apex to the base in whole mount slices. Putative carcinoma foci were morphologically verified by frozen section analysis. The fresh whole mount slices were then laid between two ceramic foam plates and fixed overnight. To test tissue preservation after this procedure, formalin-fixed and paraffin-embedded whole mount sections were stained with hematoxylin and eosin (H&E) and analyzed by immunohistochemistry, fluorescence, and silver in situ hybridization (FISH and SISH, respectively). There were no morphological artifacts on H&E stained whole mount sections from slices that had been fixed between two plates of ceramic foam, and the histological architecture was fully retained. The quality of immunohistochemistry, FISH, and SISH was excellent. Fixing whole mount tissue slices between ceramic foam plates after frozen section examination is an excellent method for processing fresh radical prostatectomy specimens, allowing for a precise identification and collection of fresh tumor tissue without compromising further diagnostic analysis.
获取前列腺癌新鲜组织对于生物样本库的建立以及异种移植模型的构建至关重要，这是晚期前列腺癌新治疗策略重要的临床前步骤。然而，鉴于前列腺组织独特的物理特性以及在大体检查中难以识别癌灶，处理新鲜的根治性前列腺切除术标本一直极具挑战性。在此，我们开发了一种新方法，使用陶瓷泡沫板处理根治性前列腺切除术标本的新鲜连续切片，且不影响进一步的诊断评估。对49个根治性前列腺切除术标本进行处理，并从尖部到基部切成连续切片。通过冷冻切片分析在形态学上验证假定的癌灶。然后将新鲜的连续切片置于两块陶瓷泡沫板之间并固定过夜。为了测试该程序后的组织保存情况，对福尔马林固定石蜡包埋的连续切片进行苏木精和伊红（H&E）染色，并通过免疫组织化学、荧光和银原位杂交（分别为FISH和SISH）进行分析。在两块陶瓷泡沫板之间固定的切片的H&E染色连续切片上没有形态学伪像，并且组织学结构完全保留。免疫组织化学、FISH和SISH的质量都很好。冷冻切片检查后将连续组织切片固定在陶瓷泡沫板之间是处理新鲜根治性前列腺切除术标本的一种极好方法，能够精确识别和收集新鲜肿瘤组织，同时不影响进一步的诊断分析。
