Lester S R, Bain J L, Serio F G, Johnson R B
Department of Periodontics and Preventive Science, University of Mississippi School of Dentistry, Jackson, MS 39216-4505, USA.
J Periodontal Res. 2009 Jun;44(3):323-9. doi: 10.1111/j.1600-0765.2008.01136.x. Epub 2008 Oct 7.
While there is substantial information concerning the concentrations of interleukin-1 isoforms within gingival crevicular fluid, there is little information concerning their concentrations within either normal or diseased gingival tissues. Therefore, the aim of this study was to evaluate the relationship between the concentrations of gingival interleukin-1 isoforms and the adjacent sulcular depth.
Interdental gingival papillae were excised and grouped based on adjacent pocket depth and the presence of bleeding on probing. Gingiva adjacent to a sulcus of < or = 3 mm without bleeding on probing were classified as 'normal'; gingiva adjacent to a 3-mm sulcus with bleeding on probing were classified as 'diseased-slight'; gingiva adjacent to a 4-6-mm sulcus featuring bleeding on probing were classified as 'diseased-moderate'; and gingiva adjacent to a sulcus of > 6 mm featuring bleeding on probing were classified as 'diseased-severe'. Tissues were solublized and the concentrations of interleukin-1beta, interleukin-1alpha, interleukin-1 receptor antagonist and interleukin-6 were assessed by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance, the post-hoc Tukey test and the Pearson's correlation test.
Gingival concentrations of interleukin-6, interleukin-1 receptor antagonist, interleukin-1alpha- and interleukin-1beta were significantly greater at diseased-severe sites than at normal, diseased-slight, or diseased-moderate sites (p < 0.05); the gingival concentrations of interleukin-1 receptor antagonist and interleukin-1alpha were significantly greater at diseased-severe than at diseased-moderate sites (p < 0.05). Interleukin-1 receptor antagonist concentrations were significantly correlated with both interleukin-1alpha and interleukin-1beta concentrations. The ratios of concentrations of the interleukin-1 isoforms were different at the various stages of inflammation.
Our data indicated a progressive increase in gingival concentrations of interleukin-1 isoforms with increased adjacent sulcular depth. However, within 'diseased' tissues, the proportional concentrations of interleukin-1alpha and -beta to interleukin-1 receptor antagonist were lowest within diseased-severe tissues.
虽然关于龈沟液中白细胞介素-1亚型的浓度已有大量信息，但关于其在正常或患病牙龈组织中的浓度信息却很少。因此，本研究的目的是评估牙龈白细胞介素-1亚型浓度与相邻龈沟深度之间的关系。
切除牙间牙龈乳头，并根据相邻牙周袋深度和探诊出血情况进行分组。探诊时相邻龈沟深度≤3mm且无出血的牙龈被分类为“正常”；探诊时相邻龈沟深度为3mm且有出血的牙龈被分类为“轻度病变”；探诊时相邻龈沟深度为4 - 6mm且有出血的牙龈被分类为“中度病变”；探诊时相邻龈沟深度>6mm且有出血且有出血的牙龈被分类为“重度病变”。将组织溶解，并通过酶联免疫吸附测定法评估白细胞介素-1β、白细胞介素-1α、白细胞介素-1受体拮抗剂和白细胞介素-6的浓度。通过方差分析、事后Tukey检验和Pearson相关检验对数据进行比较。
重度病变部位牙龈中白细胞介素-6、白细胞介素-1受体拮抗剂、白细胞介素-1α和白细胞介素-1β的浓度显著高于正常、轻度病变或中度病变部位（p<0.05）；重度病变部位牙龈中白细胞介素-1受体拮抗剂和白细胞介素-1α的浓度显著高于中度病变部位（p<0.05）。白细胞介素-1受体拮抗剂浓度与白细胞介素-1α和白细胞介素-1β浓度均显著相关。白细胞介素-1亚型的浓度比在炎症的各个阶段有所不同。
我们的数据表明，随着相邻龈沟深度增加，牙龈中白细胞介素-1亚型的浓度逐渐升高。然而，在“患病”组织中，重度病变组织中白细胞介素-1α和-β与白细胞介素-1受体拮抗剂的比例浓度最低。
