牙龈血管生成素-1浓度与相邻牙龈沟深度之间的关系。

Relationship between gingival angiopoietin-1 concentrations and depth of the adjacent gingival sulcus.

作者信息

Lester S Reid, Bain Jennifer L, Serio Francis G, Harrelson Bradley D, Johnson Roger B

机构信息

Department of Periodontics and Preventive Sciences, School of Dentistry, University of Mississippi, Jackson, MS, USA.

出版信息

J Periodontol. 2009 Sep;80(9):1447-53. doi: 10.1902/jop.2009.080643.

DOI:10.1902/jop.2009.080643

PMID:19722795

Abstract

BACKGROUND

The purpose of this study was to assess concentrations of angiopoietin (Ang)-1 at various stages of gingival inflammation. Ang-1 is an anti-inflammatory mediator present in various inflammatory diseases. However, its presence during the pathogenesis of gingival inflammation has not been established in vivo.

METHODS

Gingiva was obtained from 110 human donors before extraction of the adjacent teeth. The tissue was grouped based on adjacent probing depth and bleeding on probing (BOP). Gingiva adjacent to a <or=3-mm sulcus without BOP was classified as "normal" (N); gingiva adjacent to a 3-mm sulcus with BOP was classified as "diseased, slight" (DS); gingiva adjacent to a 4- to 6-mm sulcus featuring BOP was classified as "diseased, moderate" (DM); and gingiva adjacent to >6-mm sulci was classified as "diseased, severe" (DSev). Tissues were solublized, and concentrations of interleukin (IL)-1beta and -6, tumor necrosis factor (TNF)-alpha, endothelin (ET)-1, Ang-1, vascular cell adhesion molecule (VCAM)-1, and vascular endothelial growth factor (VEGF) were assessed by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance, the post hoc Tukey test, and the Pearson correlation test. Groups were defined as significantly different when P <0.05.

RESULTS

Gingival concentrations of IL-1beta and -6, TNF-alpha, VEGF, and ET-1 were significantly greater, and VCAM-1 and Ang-1 were significantly lower, in DSev and DM than in N and DS tissues (P <0.05). In addition, gingival concentrations of IL-6, VEGF, and ET-1 were significantly greater, and VCAM-1 and Ang-1 were significantly lower, in DSev than in DM tissues (P <0.05). There were significant positive correlations among sulcular depth, IL-1beta and -6, TNF-alpha, VEGF, and ET-1 and negative correlations among VCAM-1, Ang-1, sulcular depth, and the other biomarkers (P <0.001).

CONCLUSIONS

Depleted tissue concentrations of Ang-1 may allow gingival inflammation to become more severe because VEGF and ET-1 secretion become less inhibited. Thus, the tissues become edematous and more likely to develop BOP.

摘要

背景

本研究旨在评估血管生成素（Ang）-1在牙龈炎症各个阶段的浓度。Ang-1是一种存在于多种炎症性疾病中的抗炎介质。然而，其在牙龈炎症发病机制中的存在尚未在体内得到证实。

方法

从110名人类供体在拔除相邻牙齿前获取牙龈组织。根据相邻探诊深度和探诊出血（BOP）对组织进行分组。与≤3mm龈沟且无BOP相邻的牙龈被分类为“正常”（N）；与3mm龈沟且有BOP相邻的牙龈被分类为“患病，轻度”（DS）；与4至6mm龈沟且有BOP相邻的牙龈被分类为“患病，中度”（DM）；与>6mm龈沟相邻的牙龈被分类为“患病，重度”（DSev）。将组织溶解，并通过酶联免疫吸附测定评估白细胞介素（IL）-1β和-6、肿瘤坏死因子（TNF）-α、内皮素（ET）-1、Ang-1、血管细胞粘附分子（VCAM）-1和血管内皮生长因子（VEGF）的浓度。通过方差分析、事后Tukey检验和Pearson相关检验比较数据。当P<0.05时，组间被定义为有显著差异。

结果

与N和DS组织相比，DSev和DM组织中IL-1β和-6、TNF-α、VEGF和ET-1的牙龈浓度显著更高，而VCAM-1和Ang-1显著更低（P<0.05）。此外，与DM组织相比，DSev组织中IL-6、VEGF和ET-1的牙龈浓度显著更高，而VCAM-1和Ang-1显著更低（P<0.05）。龈沟深度、IL-1β和-6、TNF-α、VEGF和ET-1之间存在显著正相关，而VCAM-1、Ang-1、龈沟深度与其他生物标志物之间存在负相关（P<0.001）。