McGee J M, Tucci M A, Edmundson T P, Serio C L, Johnson R B
Department of Periodontics, University of Mississippi School of Dentistry, Jackson 39216-4505, USA.
J Periodontol. 1998 Aug;69(8):865-71. doi: 10.1902/jop.1998.69.8.865.
The objective of this study was to determine and compare concentrations and ratios of 3 proinflammatory cytokines, interleukin (IL) IL-1beta, IL-6, and IL-8 within gingival tissue biopsies adjacent to < or = 3, 4 to 6, or >6 mm sulci. All gingiva adjacent to > or = 4 mm sulci had clinical evidence of active inflammation. Factorial analysis of variance suggested significant effects of sulcus depth on the type and concentration of the three cytokines in the adjacent gingiva (P < 0.001). IL-8 concentrations were highest in gingiva adjacent to < or = 3 and lowest adjacent to >6 mm sulci (P < 0.001). In contrast, IL-6 concentrations were lowest in gingiva adjacent to < or = 6 mm and highest adjacent to >6 mm sites. IL-1beta concentrations were highest in gingiva adjacent to >6 mm and lowest adjacent to 4 to 6 mm sites; they were also higher adjacent to < or = 3 mm than adjacent to 4 to 6 mm sites (P < 0.01). Multiple regression analysis suggested that sulcular depth, type of cytokine, and cytokine concentration were significantly correlated (P < 0.001). Ratios of gingival cytokines changed with increased sulcular depth. In gingiva adjacent to < or = 6 mm sites, IL-8 was the most and IL-6 the least prevalent. In gingiva adjacent to > or = 6 mm sites, IL-8 was the least and IL-1-beta the most prevalent. The data suggest that the characteristics of the gingival cytokine network are affected by adjacent sulcular depth. These data could be used to design adjunct diagnostic tests for progression of periodontal diseases.
本研究的目的是测定并比较在龈沟深度≤3mm、4至6mm或＞6mm的相邻牙龈组织活检样本中3种促炎细胞因子，即白细胞介素（IL）-1β、IL-6和IL-8的浓度及比率。所有龈沟深度≥4mm的相邻牙龈均有活动性炎症的临床证据。方差分析表明龈沟深度对相邻牙龈中这三种细胞因子的类型和浓度有显著影响（P＜0.001）。IL-8浓度在龈沟深度≤3mm的相邻牙龈中最高，在＞6mm的相邻牙龈中最低（P＜0.001）。相反，IL-6浓度在龈沟深度≤6mm的相邻牙龈中最低，在＞6mm的部位最高。IL-1β浓度在＞6mm的相邻牙龈中最高，在4至6mm的部位最低；在≤3mm的相邻牙龈中也高于4至6mm的部位（P＜0.01）。多元回归分析表明龈沟深度、细胞因子类型和细胞因子浓度显著相关（P＜0.001）。牙龈细胞因子的比率随龈沟深度增加而变化。在龈沟深度≤6mm的相邻牙龈中，IL-8最为普遍，IL-6最不普遍。在龈沟深度≥6mm的相邻牙龈中，IL-8最不普遍，IL-1-β最为普遍。这些数据表明牙龈细胞因子网络的特征受相邻龈沟深度的影响。这些数据可用于设计牙周疾病进展的辅助诊断测试。
