Tasaka K, Mio M, Akagi M, Fujisawa K, Aoki I
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Agents Actions. 1991 May;33(1-2):44-7. doi: 10.1007/BF01993123.
In order to study the role of the cytoskeleton in histamine release from mast cells, the effects of cytochalasin D, cholchicine and vinblastine on Ca2+ release from the intracellular Ca store induced by compound 48/80 were investigated by means of a video-intensified microscopy system. When the quin 2-loaded mast cells were stimulated by 0.35 micrograms/ml of compound 48/80, a rapid increase in intracellular Ca2+ was observed. At concentrations higher than 10(-6) M, both colchicine and vinblastine pretreatments significantly inhibited the increase in intracellular Ca2+ concentrations caused by compound 48/80, although cytochalasin D had no effect. When permeabilized mast cells were exposed to potassium-antimonate solution, microtubules became attached to the endoplasmic reticulum, where many dots of Ca-antimonate were observed; in some areas, the microtubules interconnected the endoplasmic reticulum and granules in the mast cells. From the results of the present study, it was assumed that microtubules play some important role in the processes leading to Ca2+ release from the intracellular Ca store.
为了研究细胞骨架在肥大细胞组胺释放中的作用，采用视频增强显微镜系统研究了细胞松弛素D、秋水仙碱和长春碱对化合物48/80诱导的细胞内钙库释放Ca2+的影响。当用0.35微克/毫升的化合物48/80刺激负载喹啉-2的肥大细胞时，观察到细胞内Ca2+迅速增加。在高于10^(-6) M的浓度下，秋水仙碱和长春碱预处理均显著抑制了化合物48/80引起的细胞内Ca2+浓度升高，而细胞松弛素D则无此作用。当通透的肥大细胞暴露于钾锑酸盐溶液中时，微管附着在内质网上，在内质网上观察到许多锑酸钙点；在某些区域，微管将内质网与肥大细胞中的颗粒相互连接。从本研究结果推测，微管在导致细胞内钙库释放Ca2+的过程中起重要作用。
