Mio M, Tasaka K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Int Arch Allergy Appl Immunol. 1989;88(3):369-71. doi: 10.1159/000234829.
When compound 48/80 was applied by means of microelectrophoresis to the surface of a rat peritoneal mast cell, localized degranulation was observed in the area close to the microelectrode tip. The extruded granules were connected to the cell surface by filaments. The filaments were elongated radially and, in some occasions, projected to a length of 5 microns. A few minutes later, the length of the protruded filaments became shorter and shorter and, finally, the extruded granules were reincorporated into the cell. When rhodamine-phalloidin, an F-actin-specific dye, was perfused the extruded granules and filaments were stained by this dye. This indicates that actin filaments or fragments of them exist on the granule surface and on the cell surface at the site of degranulation. These actin filaments bound to the mast cell granules may play an important role, not only for the extrusion of the granules, but also for the reuptake of extruded granules into the cytoplasm.
当通过微电泳将化合物48/80施加到大鼠腹膜肥大细胞表面时，在靠近微电极尖端的区域观察到局部脱颗粒现象。挤出的颗粒通过细丝与细胞表面相连。细丝呈放射状伸长，在某些情况下，伸出长度可达5微米。几分钟后，伸出细丝的长度变得越来越短，最终，挤出的颗粒重新并入细胞。当用罗丹明 - 鬼笔环肽（一种F - 肌动蛋白特异性染料）灌注时，挤出的颗粒和细丝被这种染料染色。这表明肌动蛋白丝或其片段存在于脱颗粒部位的颗粒表面和细胞表面。这些与肥大细胞颗粒结合的肌动蛋白丝可能不仅对颗粒的挤出起重要作用，而且对挤出颗粒重新摄取到细胞质中也起重要作用。
