Rallapalli S, Verghese S, Verma R S
Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India.
Indian J Med Microbiol. 2008 Oct-Dec;26(4):361-4. doi: 10.4103/0255-0857.43580.
Multiplex polymerase chain reaction (PCR) strategy is described for rapid identification of clinically relevant methicillin resistant Staphylococcus aureus (MRSA) that targets mecA and coagulase genes. In this study, 150 staphylococcal clinical isolates were used that included 40 isolates of MRSA, 55 isolates of methicillin susceptible S. aureus (MSSA), 44 isolates of methicillin susceptible coagulase negative Staphylococcus spp. (MS-CoNS) and 11 isolates of methicillin resistant coagulase negative Staphylococcus spp. (MR-CoNS). Out of 55 S. aureus strains, three strains demonstrated mecA gene, which appeared to be oxacillin sensitive by disc diffusion. When (MS-CoNS) were evaluated, 10 isolates classified as oxacillin sensitive phenotypically, yielded positive results in PCR method. The results for mecA detection by PCR were more consistent with disk susceptibility tests in case of MRSA (100%) and MSSA (95%) isolates. In contrast to above results with MRSA and MSSA, mecA detection by PCR in MS-CoNS showed less correlation with disk susceptibility tests (77%). The results for coag detection by PCR were consistent with phenotypic tests in all isolates.
描述了一种多重聚合酶链反应（PCR）策略，用于快速鉴定针对mecA和凝固酶基因的临床相关耐甲氧西林金黄色葡萄球菌（MRSA）。在本研究中，使用了150株葡萄球菌临床分离株，其中包括40株MRSA分离株、55株甲氧西林敏感金黄色葡萄球菌（MSSA）分离株、44株甲氧西林敏感凝固酶阴性葡萄球菌属（MS-CoNS）分离株和11株耐甲氧西林凝固酶阴性葡萄球菌属（MR-CoNS）分离株。在55株金黄色葡萄球菌菌株中，有3株显示出mecA基因，通过纸片扩散法检测似乎对苯唑西林敏感。在评估（MS-CoNS）时，10株表型分类为苯唑西林敏感的分离株，PCR方法检测结果呈阳性。对于MRSA（100%）和MSSA（95%）分离株，PCR检测mecA的结果与纸片药敏试验更一致。与MRSA和MSSA的上述结果相反，MS-CoNS中PCR检测mecA与纸片药敏试验的相关性较低（77%）。PCR检测凝固酶的结果与所有分离株的表型试验一致。
