Detection and phenotypic characterization of methicillin-resistant from clinical and community samples in Abakaliki, Ebonyi State, Nigeria.

OBJECTIVE

The objective of this study was to isolate and phenotypically characterize methicillin-resistant (MRSA)from clinical and community samples in Abakaliki, Nigeria.

METHODS

A total of 709 clinical (303) and community samples (406) samples were obtained for this study. MRSA isolates were detected using Kirby-Bauer disc diffusion method with the inclusion of 1 µg oxacillin and cefoxitin antibiotic disc. The isolates were screened for the β-lactamase production using nitrocefin sticks.

RESULTS

A total of 44 MRSA isolates were obtained from the samples with prevalence frequency of 22.6 % and 20.8 % from clinical and community samples respectively. The clinical isolates were completely resistant (100 %) to ceftazidime, tetracycline and penicillin. Gentamicin and ciprofloxacin were the most effective antibiotics against the clinical and community isolates respectively with a susceptibility frequency of 63.2 % and 80 %. Exactly 38.1 % and 24.2 % of the clinical and community isolates were positive for beta-lactamase production respectively. The HA-MRSA and CA-MRSA isolates had MARI values within the range of 0.3 to 1.0.

CONCLUSION

This present findings of multi-drug resistance MRSA is very worrisome as it further highlights the pressing need to keep a strict watch on MRSA emerging from this study area.