Ariom T O, Iroha I R, Moses I B, Iroha C S, Ude U I, Kalu A C
Department of Applied Microbiology, Ebonyi State University, Faculty of Sciences, Ebonyi State University, Abakaliki.
Department of Pharmacy, Federal Teaching Hospital, Abakaliki.
Afr Health Sci. 2019 Jun;19(2):2026-2035. doi: 10.4314/ahs.v19i2.26.
The objective of this study was to isolate and phenotypically characterize methicillin-resistant (MRSA)from clinical and community samples in Abakaliki, Nigeria.
A total of 709 clinical (303) and community samples (406) samples were obtained for this study. MRSA isolates were detected using Kirby-Bauer disc diffusion method with the inclusion of 1 µg oxacillin and cefoxitin antibiotic disc. The isolates were screened for the β-lactamase production using nitrocefin sticks.
A total of 44 MRSA isolates were obtained from the samples with prevalence frequency of 22.6 % and 20.8 % from clinical and community samples respectively. The clinical isolates were completely resistant (100 %) to ceftazidime, tetracycline and penicillin. Gentamicin and ciprofloxacin were the most effective antibiotics against the clinical and community isolates respectively with a susceptibility frequency of 63.2 % and 80 %. Exactly 38.1 % and 24.2 % of the clinical and community isolates were positive for beta-lactamase production respectively. The HA-MRSA and CA-MRSA isolates had MARI values within the range of 0.3 to 1.0.
This present findings of multi-drug resistance MRSA is very worrisome as it further highlights the pressing need to keep a strict watch on MRSA emerging from this study area.
本研究的目的是从尼日利亚阿巴卡利基的临床和社区样本中分离耐甲氧西林金黄色葡萄球菌（MRSA）并进行表型特征分析。
本研究共采集了709份临床样本（303份）和社区样本（406份）。采用Kirby-Bauer纸片扩散法检测MRSA分离株，其中包括1μg苯唑西林和头孢西丁抗生素纸片。使用硝基头孢菌素试纸条筛选分离株的β-内酰胺酶产生情况。
从样本中总共获得了44株MRSA分离株，临床样本和社区样本的流行频率分别为22.6%和20.8%。临床分离株对头孢他啶、四环素和青霉素完全耐药（100%）。庆大霉素和环丙沙星分别是对临床和社区分离株最有效的抗生素，敏感频率分别为63.2%和80%。临床和社区分离株中分别有38.1%和24.2%的β-内酰胺酶产生呈阳性。医院获得性MRSA（HA-MRSA）和社区获得性MRSA（CA-MRSA）分离株的MARI值在0.3至1.0范围内。
本研究中耐多药MRSA的发现令人十分担忧，因为这进一步凸显了对该研究区域出现的MRSA进行严格监测的迫切需求。
