Heck Albert J R
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
Nat Methods. 2008 Nov;5(11):927-33. doi: 10.1038/nmeth.1265.
Native mass spectrometry is an emerging technology that allows the topological investigation of intact protein complexes with high sensitivity and a theoretically unrestricted mass range. This unique tool provides complementary information to established technologies in structural biology, and also provides a link to high-throughput interactomics studies, which do not generate information on exact protein complex-composition, structure or dynamics. Here I review the current state of native mass spectrometry technology and discuss several important biological applications. I also describe current experimental challenges in native mass spectrometry, encouraging readers to contribute to solutions.
原生质谱是一项新兴技术，它能够以高灵敏度和理论上无限制的质量范围对完整的蛋白质复合物进行拓扑学研究。这一独特工具为结构生物学中的现有技术提供了补充信息，还为高通量相互作用组学研究建立了联系，高通量相互作用组学研究无法生成有关精确蛋白质复合物组成、结构或动力学的信息。在此，我回顾了原生质谱技术的当前状态，并讨论了几个重要的生物学应用。我还描述了原生质谱当前面临的实验挑战，鼓励读者为解决这些问题贡献力量。
