Granzotto Marilena, dal Bo Sara, Quaglia Sara, Tommasini Alberto, Piscianz Elisa, Valencic Erica, Ferrara Fortunato, Martelossi Stefano, Ventura Alessandro, Not Tarcisio
Laboratory of Immunology, IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34137, Trieste, Italy.
Dig Dis Sci. 2009 Jul;54(7):1513-9. doi: 10.1007/s10620-008-0501-x. Epub 2008 Oct 31.
Celiac disease (CD) is characterized by intolerance to gluten and high risk of developing autoimmune phenomena. Possible defects in immune tolerance could have a role in the pathogenesis of the disease. As regulatory T-cells (Tregs) are the main population involved in maintaining peripheral tolerance, we investigated the number of these cells in celiac patients as compared with healthy donors. Moreover, we analyzed the suppressive function of CD4+CD25+ T-cells from celiac disease patients and controls on autologous responder T-cells (CD4+CD25-). The percentage of CD4+CD25+FOXP3+ cells was not different in celiacs and in healthy controls, and among positive cells the level of expression of the two regulatory markers was comparable. However, the suppressor activity of Tregs was significantly impaired in CD patients. These results suggest that a defect in Tregs function could play a role in the pathogenesis of CD and in CD-associated autoimmunity.
乳糜泻（CD）的特征是对麸质不耐受以及发生自身免疫现象的风险较高。免疫耐受方面可能存在的缺陷可能在该疾病的发病机制中起作用。由于调节性T细胞（Tregs）是参与维持外周耐受的主要细胞群体，我们研究了与健康供体相比，乳糜泻患者中这些细胞的数量。此外，我们分析了乳糜泻患者和对照的CD4 + CD25 + T细胞对自体反应性T细胞（CD4 + CD25 -）的抑制功能。CD4 + CD25 + FOXP3 +细胞的百分比在乳糜泻患者和健康对照中没有差异，并且在阳性细胞中，两种调节标志物的表达水平相当。然而，CD患者中Tregs的抑制活性明显受损。这些结果表明，Tregs功能缺陷可能在CD的发病机制以及与CD相关的自身免疫中起作用。
