Courtright J B
Biochem Genet. 1976 Dec;14(11-12):1057-63. doi: 10.1007/BF00485136.
The inducible cytosolic glycerokinase and mitochondrial glycerol-3-phosphate dehydrogenase have been examined during the glycerol-specific induction in Neurospora crassa. Although both the fully induced levels and the respective rates of synthesis of these two enzymes were less than observed with wild-type cells, there were no major differences in the relative rates of induction of the glycerol-3-phosphate dehydrogenase at either permissive or restrictive temperatures. These results indicate that the processes involved in the assembly of this enzyme into the mitochondrial inner membrane are normal in a mutant lacking the mitochondrial leucyl tRNA synthetase and suggest that the functions of the mitochondrial synthetase may be replaced by those of the cytosolic leucyl tRNA synthetase.
在粗糙脉孢菌的甘油特异性诱导过程中,对诱导型胞质甘油激酶和线粒体甘油-3-磷酸脱氢酶进行了检测。尽管这两种酶的完全诱导水平和各自的合成速率均低于野生型细胞,但在允许温度或限制温度下,甘油-3-磷酸脱氢酶的相对诱导速率并无显著差异。这些结果表明,在缺乏线粒体亮氨酰tRNA合成酶的突变体中,该酶组装到线粒体内膜的过程是正常的,这表明线粒体合成酶的功能可能被胞质亮氨酰tRNA合成酶的功能所取代。