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粗糙脉孢菌leu-5突变体中磷酸甘油途径酶的诱导作用。

Induction of enzymes of the glycerophosphate pathway in leu-5 mutants of Neurospora crassa.

作者信息

Courtright J B

出版信息

Biochem Genet. 1976 Dec;14(11-12):1057-63. doi: 10.1007/BF00485136.

DOI:10.1007/BF00485136
PMID:189751
Abstract

The inducible cytosolic glycerokinase and mitochondrial glycerol-3-phosphate dehydrogenase have been examined during the glycerol-specific induction in Neurospora crassa. Although both the fully induced levels and the respective rates of synthesis of these two enzymes were less than observed with wild-type cells, there were no major differences in the relative rates of induction of the glycerol-3-phosphate dehydrogenase at either permissive or restrictive temperatures. These results indicate that the processes involved in the assembly of this enzyme into the mitochondrial inner membrane are normal in a mutant lacking the mitochondrial leucyl tRNA synthetase and suggest that the functions of the mitochondrial synthetase may be replaced by those of the cytosolic leucyl tRNA synthetase.

摘要

在粗糙脉孢菌的甘油特异性诱导过程中,对诱导型胞质甘油激酶和线粒体甘油-3-磷酸脱氢酶进行了检测。尽管这两种酶的完全诱导水平和各自的合成速率均低于野生型细胞,但在允许温度或限制温度下,甘油-3-磷酸脱氢酶的相对诱导速率并无显著差异。这些结果表明,在缺乏线粒体亮氨酰tRNA合成酶的突变体中,该酶组装到线粒体内膜的过程是正常的,这表明线粒体合成酶的功能可能被胞质亮氨酰tRNA合成酶的功能所取代。

相似文献

1
Induction of enzymes of the glycerophosphate pathway in leu-5 mutants of Neurospora crassa.粗糙脉孢菌leu-5突变体中磷酸甘油途径酶的诱导作用。
Biochem Genet. 1976 Dec;14(11-12):1057-63. doi: 10.1007/BF00485136.
2
Differential rates of synthesis of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassa during induction.
Arch Biochem Biophys. 1975 Mar;167(1):34-44. doi: 10.1016/0003-9861(75)90438-5.
3
Biochemical comparison of the Neurospora crassa wild type and the temperature-sensitive and leucine-auxotroph mutant leu-5. Purification of the cytoplasmic and mitochondrial leucyl-tRNA synthetases and comparison of the enzymatic activities and the degradation patterns.粗糙脉孢菌野生型与温度敏感型及亮氨酸营养缺陷型突变体leu-5的生化比较。细胞质和线粒体亮氨酰-tRNA合成酶的纯化以及酶活性和降解模式的比较。
Eur J Biochem. 1986 Jul 1;158(1):43-9. doi: 10.1111/j.1432-1033.1986.tb09718.x.
4
Proposed involvement of an internal promoter in regulation and synthesis of mitochondrial and cytoplasmic leucyl-tRNA synthetases of Neurospora.关于内部启动子参与粗糙脉孢菌线粒体和细胞质亮氨酰 - tRNA合成酶调控与合成的推测。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1172-6. doi: 10.1073/pnas.74.3.1172.
5
Biochemical comparison of the Neurospora crassa wild-type and the temperature-sensitive leucine-auxotroph mutant leu-5. Detailed kinetic comparison of the leucyl-tRNA synthetases.粗糙脉孢菌野生型与温度敏感型亮氨酸营养缺陷型突变体leu-5的生化比较。亮氨酰-tRNA合成酶的详细动力学比较。
Eur J Biochem. 1986 Jul 1;158(1):51-6. doi: 10.1111/j.1432-1033.1986.tb09719.x.
6
Regulation of cytoplasmic and mitochondrial leucyl-transfer ribonucleic acid synthetases in Neurospora crassa.粗糙脉孢菌中细胞质和线粒体亮氨酰转移核糖核酸合成酶的调控
J Bacteriol. 1980 Oct;144(1):447-50. doi: 10.1128/jb.144.1.447-450.1980.
7
Intracellular localization and properties of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassa.粗糙脉孢菌中甘油激酶和甘油磷酸脱氢酶的细胞内定位及特性
Arch Biochem Biophys. 1975 Mar;167(1):21-33. doi: 10.1016/0003-9861(75)90437-3.
8
Nuclear gene for mitochondrial leucyl-tRNA synthetase of Neurospora crassa: isolation, sequence, chromosomal mapping, and evidence that the leu-5 locus specifies structural information.粗糙脉孢菌线粒体亮氨酰 - tRNA合成酶的核基因:分离、序列、染色体定位以及亮氨酸-5位点指定结构信息的证据
Mol Cell Biol. 1989 Nov;9(11):4631-44. doi: 10.1128/mcb.9.11.4631-4644.1989.
9
Genetic and enzymatic characterization of the inducible glycerol dissimilatory system of Neurospora crassa.粗糙脉孢菌诱导型甘油异化系统的遗传与酶学特性
J Bacteriol. 1982 Aug;151(2):912-7. doi: 10.1128/jb.151.2.912-917.1982.
10
Regulation of the nuclear genes encoding the cytoplasmic and mitochondrial leucyl-tRNA synthetases of Neurospora crassa.粗糙脉孢菌胞质和线粒体亮氨酰 - tRNA合成酶核基因的调控。
Mol Cell Biol. 1989 Nov;9(11):4645-52. doi: 10.1128/mcb.9.11.4645-4652.1989.

引用本文的文献

1
Chromosomal loci of Neurospora crassa.粗糙脉孢菌的染色体位点。
Microbiol Rev. 1982 Dec;46(4):426-570. doi: 10.1128/mr.46.4.426-570.1982.
2
Isolation and characterization of glycerol-3-phosphate dehydrogenase-defective mutants of Neurospora crassa.粗糙脉孢菌甘油-3-磷酸脱氢酶缺陷型突变体的分离与鉴定。
J Bacteriol. 1978 Dec;136(3):960-8. doi: 10.1128/jb.136.3.960-968.1978.

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Histidyl-tRNAs and histidyl-tRNA synthetases in wild type and cytoplasmic petite mutants of Saccharomyces cerevisiae.酿酒酵母野生型和细胞质小菌落突变体中的组氨酰 - tRNA 及组氨酰 - tRNA 合成酶
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7
Precursors of cytochrome oxidase in cytochrome-oxidase-deficient cells of Neurospora crassa. Comparison of the nuclear mutant cni-1, the cytoplasmic mutant mi-1, and copper-depleted wild type.粗糙脉孢菌细胞色素氧化酶缺陷型细胞中细胞色素氧化酶的前体。细胞核突变体cni-1、细胞质突变体mi-1与缺铜野生型的比较。
Eur J Biochem. 1974 Dec 2;49(3):607-17. doi: 10.1111/j.1432-1033.1974.tb03864.x.
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Inhibition of the assembly of cytochrome oxidase in Neurospora crassa by chloramphenicol.氯霉素对粗糙脉孢菌中细胞色素氧化酶组装的抑制作用。
Eur J Biochem. 1972 Nov 7;30(3):413-7. doi: 10.1111/j.1432-1033.1972.tb02112.x.
9
Assesmbly of the mitochondrial membrane system. VI. Mitochondrial synthesis of subunit proteins of the rutamycin-sensitive adenosine triphosphatase.线粒体膜系统的组装。VI. 对鲁塔霉素敏感的三磷酸腺苷酶亚基蛋白的线粒体合成。
J Biol Chem. 1972 Jan 25;247(2):594-603.
10
Intracellular localization and properties of glycerokinase and glycerophosphate dehydrogenase in Neurospora crassa.粗糙脉孢菌中甘油激酶和甘油磷酸脱氢酶的细胞内定位及特性
Arch Biochem Biophys. 1975 Mar;167(1):21-33. doi: 10.1016/0003-9861(75)90437-3.