Tsui Florence W L, Xi Nancy, Rohekar Sherry, Riarh Reena, Bilotta Rose, Tsui Hing Wo, Inman Robert D
Toronto Western Research Institute, University Health Network, and University of Toronto, Toronto, Ontario, Canada.
Arthritis Rheum. 2008 Nov;58(11):3436-8. doi: 10.1002/art.23967.
We previously reported a recent outbreak of salmonellosis in which some individuals developed complications of the enteric infection. The objective of this study was to identify genetic variants that might predispose infected individuals to develop articular and/or extraarticular sequelae after Salmonella enteritidis infection.
The entire exposed cohort was invited to participate in the study by sending a saliva sample for DNA analysis. Seventy-five Salmonella-infected subjects for whom there was clinical information agreed to participate and were stratified into 4 groups. Group 1 patients had arthritis and extraarticular features, group 2 patients had arthritis alone, group 3 patients had extraarticular features alone, and group 4 patients had neither. DNA samples from an uninfected cohort of 91 normal subjects were also genotyped. Genotyping was performed using 2 Toll-like receptor 2 (TLR-2) (rs5743708 and rs5743704) and 2 TLR-4 (rs4986790 and rs4986791) single-nucleotide polymorphisms (SNPs). Statistical analyses were carried out using chi-square tests.
There was no association of TLR-4 exonic variants with any clinical events that were reported as accompanying the Salmonella infection. In contrast, compared with normal controls, one of the rare TLR-2 SNPs (rs5743708, R753Q) was associated with the development of arthritis and extraarticular features (P = 0.015 by chi-square test). The TLR-2 variant 753Q was not detected in any of the infected individuals with an uncomplicated course. Another TLR-2 variant, 631H, was associated with articular symptoms in infected males (P = 0.03 by chi-square test).
In this outbreak, genetic variants of TLR-2, but not TLR-4, were associated with acute reactive arthritis following infection with S enteritidis.
我们之前报道了近期一起沙门氏菌病暴发事件，其中一些个体出现了肠道感染并发症。本研究的目的是确定可能使感染个体在感染肠炎沙门氏菌后易发生关节和/或关节外后遗症的基因变异。
通过寄送唾液样本进行DNA分析，邀请整个暴露队列参与研究。75名有临床信息的沙门氏菌感染受试者同意参与并被分为4组。第1组患者有关节炎和关节外表现，第2组患者仅有关节炎，第3组患者仅有关节外表现，第4组患者两者均无。还对91名正常受试者的未感染队列的DNA样本进行了基因分型。使用2个Toll样受体2（TLR - 2）（rs5743708和rs5743704）和2个TLR - 4（rs4986790和rs4986791）单核苷酸多态性（SNP）进行基因分型。使用卡方检验进行统计分析。
TLR - 4外显子变异与报道的伴随沙门氏菌感染的任何临床事件均无关联。相比之下，与正常对照组相比，一种罕见的TLR - 2 SNP（rs5743708，R753Q）与关节炎和关节外表现的发生相关（卡方检验P = 0.015）。在任何病程无并发症的感染个体中均未检测到TLR - 2变异体753Q。另一种TLR - 2变异体631H与感染男性的关节症状相关（卡方检验P = 0.03）。
在此次暴发中，TLR - 2的基因变异而非TLR - 4的基因变异与肠炎沙门氏菌感染后的急性反应性关节炎相关。
