大鼠促黄体素（促黄体生成素）卵巢受体的研究。放射免疫测定法在测量与受体结合的促黄体素方面的适用性。
Studies on rat ovarian receptors for lutropin (luteinizing hormone). Applicability of radioimmunoassay to measure lutropin bound to receptors.
作者信息
Muralidhar K, Moudgal N R
出版信息
Biochem J. 1976 Dec 15;160(3):603-6. doi: 10.1042/bj1600603.
Abstract
Measurement of receptor-bound unlabelled physiologically active lutropin (luteinizing hormone, LH) was possible by a modified radioimmunoassay. The conventional radioimmunoassay conducted at 4 degrees C was inadequate, whereas the modified assay performed at 37 degrees C could measure receptor-bound lutropin. The radioimmunoassay at 37 degrees C takes only 36h for completion compared with 5-7 days at 4 degreesC. The sensitivity and range of dose-response curves are, however, unaltered. The validity of the technique was established by a number of criteria.
摘要
通过一种改良的放射免疫测定法能够测量与受体结合的未标记生理活性促黄体激素（促性腺激素，LH）。在4摄氏度下进行的传统放射免疫测定法并不充分，而在37摄氏度下进行的改良测定法能够测量与受体结合的促黄体激素。与在4摄氏度下需要5至7天相比，在37摄氏度下的放射免疫测定法仅需36小时就能完成。然而，剂量反应曲线的灵敏度和范围并未改变。该技术的有效性通过多项标准得以确立。
相似文献
1
Studies on rat ovarian receptors for lutropin (luteinizing hormone). Applicability of radioimmunoassay to measure lutropin bound to receptors.大鼠促黄体素（促黄体生成素）卵巢受体的研究。放射免疫测定法在测量与受体结合的促黄体素方面的适用性。
Biochem J. 1976 Dec 15;160(3):603-6. doi: 10.1042/bj1600603.
2
Studies on rat ovarian receptors for lutropin (luteinizing hormone). Interaction with beta-subunit of sheep lutropin.大鼠促黄体素（促性腺激素）卵巢受体的研究。与绵羊促黄体素β亚基的相互作用。
Biochem J. 1976 Dec 15;160(3):615-9. doi: 10.1042/bj1600615.
3
Studies on rat ovarian receptors for lutropin (luteinizing hormone). Factors influencing binding and response.大鼠促黄体素（黄体生成素）卵巢受体的研究。影响结合及反应的因素。
Biochem J. 1976 Dec 15;160(3):607-13. doi: 10.1042/bj1600607.
4
Gonadal luteinizing hormone receptors and adenylate cyclase: transfer of functional ovarian luteinizing hormone receptors to adrenal fasciculata cells.性腺促黄体生成素受体与腺苷酸环化酶：功能性卵巢促黄体生成素受体向肾上腺束状带细胞的转移。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4769-73. doi: 10.1073/pnas.75.10.4769.
5
Rabbit lutropin: preparation, characterization of the hormone, its subunits and radioimmunoassay.兔促黄体激素：激素及其亚基的制备、特性鉴定与放射免疫测定
Int J Pept Protein Res. 1979 Jan;13(1):62-70. doi: 10.1111/j.1399-3011.1979.tb01850.x.
6
Lutropin receptors from male and female tissues: different responses to a lutropin receptor binding inhibitor.
Proc Soc Exp Biol Med. 1976 Sep;152(4):606-9. doi: 10.3181/00379727-152-39450.
7
[Validation of the radioimmunoassay for rat luteinizing hormone (LH) (author's transl)].大鼠促黄体生成素（LH）放射免疫测定法的验证（作者译）
Reproduccion. 1980 Apr-Jun;4(2):109-33.
8
Ability of human chorionic gonadotropin beta-subunit to inhibit the steroidogenic response to lutropin.人绒毛膜促性腺激素β亚基抑制对促黄体激素的类固醇生成反应的能力。
Biochem J. 1979 Jun 15;180(3):573-8. doi: 10.1042/bj1800573.
9
Prolactin involvement in the maintenance of ovarian lutropin receptors in lactating rat.
Indian J Exp Biol. 1980 Mar;18(3):327-8.
10
Changes in ovarian luteinizing hormone and follicle-stimulating hormone receptor content and in gonadotropin-induced ornithine decarboxylase activity during prepubertal and pubertal development of the female rat.雌性大鼠青春期前和青春期发育过程中卵巢促黄体生成素和促卵泡激素受体含量以及促性腺激素诱导的鸟氨酸脱羧酶活性的变化。
Endocrinology. 1981 Jul;109(1):152-61. doi: 10.1210/endo-109-1-152.
引用本文的文献
1
Ability of human chorionic gonadotropin beta-subunit to inhibit the steroidogenic response to lutropin.人绒毛膜促性腺激素β亚基抑制对促黄体激素的类固醇生成反应的能力。
Biochem J. 1979 Jun 15;180(3):573-8. doi: 10.1042/bj1800573.
2
Studies on rat ovarian receptors for lutropin (luteinizing hormone). Interaction with beta-subunit of sheep lutropin.大鼠促黄体素（促性腺激素）卵巢受体的研究。与绵羊促黄体素β亚基的相互作用。
Biochem J. 1976 Dec 15;160(3):615-9. doi: 10.1042/bj1600615.
3
Studies on rat ovarian receptors for lutropin (luteinizing hormone). Factors influencing binding and response.大鼠促黄体素（黄体生成素）卵巢受体的研究。影响结合及反应的因素。
Biochem J. 1976 Dec 15;160(3):607-13. doi: 10.1042/bj1600607.
本文引用的文献
1
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
2
Specific binding of luteinizing hormone to Leydig tumor cells.促黄体生成素与睾丸间质细胞瘤细胞的特异性结合。
J Biol Chem. 1971 Aug 25;246(16):4983-6.
3
The uptake of human luteinizing hormone (hLH) by slices of luteinized rat ovaries.黄体化大鼠卵巢切片对人促黄体生成素（hLH）的摄取。
Endocrinology. 1971 Dec;89(6):1515-23. doi: 10.1210/endo-89-6-1515.
4
Specific receptor for LH in the ovary: evidence by autoradiography and tissue fractionation.卵巢中促黄体生成素的特异性受体：放射自显影和组织分级分离证据
Endocrinology. 1972 Jan;90(1):1-9. doi: 10.1210/endo-90-1-1.
5
Enzymatic radioiodination of gonadotropins.促性腺激素的酶促放射性碘化
J Clin Endocrinol Metab. 1972 Jan;34(1):23-8. doi: 10.1210/jcem-34-1-23.
6
Hormonal control of gestation in the intact rat.完整大鼠妊娠期的激素调控
Endocrinology. 1970 Apr;86(4):874-89. doi: 10.1210/endo-86-4-874.
7
An improved method for the preparation of iodinated antigens for radioimmunoassay.一种改进的用于放射免疫分析的碘化抗原制备方法。
J Endocrinol. 1974 Mar;60(3):527-8. doi: 10.1677/joe.0.0600527.
8
The conversion of acetate to cholesterol in the fetus of the baboon and the transfer of cholesterol from mother to fetus.狒狒胎儿中乙酸盐向胆固醇的转化以及胆固醇从母体向胎儿的转移。
Endocrinology. 1972 Jul;91(1):6-12. doi: 10.1210/endo-91-1-6.
9
Membrane receptors.膜受体
Annu Rev Biochem. 1974;43(0):169-214. doi: 10.1146/annurev.bi.43.070174.001125.
10
Characterization and solubilization of gonadotropin receptor of bovine corpus luteum.
J Biol Chem. 1974 Apr 10;249(7):2195-205.
Zotero 插件