Gao Jun-jie, Zhang Lin, Qin Ai-guo, Yu Xian-chang
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai' an 271018, Shandong, China.
Ying Yong Sheng Tai Xue Bao. 2008 Aug;19(8):1754-8.
Taking grafted and own-root cucumber (Junlv No. 3) plants as test materials, the changes of their leaf Cu/Zn-SOD, Mn-SOD and CAT mRNA relative expression, leaf SOD, Cu/Zn-SOD, Mn-SOD and CAT activities, leaf MDA content, and leaf electrolytic leakage under NaCI stress were studied. The results showed that under NaCl stress, grafted cucumber had a higher relative expression of its leaf Cu/Zn-SOD, Mn-SOD and CAT mRNA, and higher activities of its leaf SOD, Cu/Zn-SOD, Mn-SOD and CAT, compared with own-root cucumber, which suggested that the higher relative expression of Cu/Zn-SOD, Mn-SOD and CAT mRNA in grafted cucumber leaves resulted in the higher activities of their SOD, Cu/Zn-SOD, Mn-SOD and CAT. With the NaCl stress prolonged, the relative expression of Cu/Zn-SOD, Mn-SOD and CAT mRNA in both grafted and own-root cucumber leaves had an increasing trend, but the changing trend of Cu/Zn-SOD, MnSOD and CAT activities were not the same as that of mRNA, indicating that the activities of SOD and CAT were also regulated by other factors. The leaf MDA content and electrolytic leakage of grafted cucumber were lower than those of own-root cucumber, suggesting that grafted cucumber had a higher scavenging metabolism, which could decrease oxidative damage and increase its salt-tolerance.
以嫁接黄瓜和自根黄瓜（津绿3号）植株为试验材料，研究了NaCl胁迫下其叶片Cu/Zn-SOD、Mn-SOD和CAT mRNA相对表达量、叶片SOD、Cu/Zn-SOD、Mn-SOD和CAT活性、叶片MDA含量以及叶片电解质渗透率的变化。结果表明，在NaCl胁迫下，与自根黄瓜相比，嫁接黄瓜叶片Cu/Zn-SOD、Mn-SOD和CAT mRNA相对表达量较高，叶片SOD、Cu/Zn-SOD、Mn-SOD和CAT活性也较高，这表明嫁接黄瓜叶片中Cu/Zn-SOD、Mn-SOD和CAT mRNA相对表达量较高导致了其SOD、Cu/Zn-SOD、Mn-SOD和CAT活性较高。随着NaCl胁迫时间的延长，嫁接黄瓜和自根黄瓜叶片中Cu/Zn-SOD、Mn-SOD和CAT mRNA相对表达量均呈上升趋势，但Cu/Zn-SOD、Mn-SOD和CAT活性的变化趋势与mRNA不同，表明SOD和CAT的活性还受其他因素调控。嫁接黄瓜叶片MDA含量和电解质渗透率低于自根黄瓜，表明嫁接黄瓜具有较高的清除代谢能力，可减少氧化损伤并提高其耐盐性。
