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[黄瓜幼苗对NaCl和NaHCO₃胁迫的生理响应差异]

[Differences of physiological responses of cucumber seedlings to NaCl and NaHCO3 stress].

作者信息

Zhang Li-ping, Wang Xiu-feng, Shi Qing-hua, Gao Qing-hai, Liu Ze-zhou

机构信息

College of of Horticulture Science and Engineering, Shandong Agricultural University Tai' an 271018, Shandong, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2008 Aug;19(8):1854-9.

Abstract

By a solution culture experiment, cucumber seedlings were treated with different concentration (0, 25, 50 and 75 mmol x L(-1)) NaCl and NaHCO3, and their physiological responses were studied. The results showed that with increasing NaCl and NaHCO3 stress, the shoot and root growth, the leaf chlorophyll content and relative water content of the seedlings decreased dramatically, and the decrements were greater under NaHCO3 than under NaCl stress. The shoot Na content increased significantly with increasing NaCl and NaHCO, stress, while shoot K+ content was in adverse. At the same concentration of Na+ in culture solution, the addition of NaHCO3 induced a greater decrement of shoot K+ content, and thus, a lower shoot K+/Na+ ratio. Under NaHCO3 stress, the leaf electrolyte leakage rate and the leaf MDA, proline, and soluble sugar contents increased much greater, compared with those under NaCl stress. Under NaCl and NaHCO3 stress, the leaf superoxide dismutase (SOD), ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR) activities were induced significantly, while the leaf peroxidase (POD) activity was inhibited obviously.

摘要

通过溶液培养试验,用不同浓度(0、25、50和75 mmol·L⁻¹)的NaCl和NaHCO₃处理黄瓜幼苗,并研究其生理响应。结果表明,随着NaCl和NaHCO₃胁迫的增加,幼苗的地上部和根系生长、叶片叶绿素含量和相对含水量显著下降,且在NaHCO₃胁迫下的下降幅度大于NaCl胁迫。随着NaCl和NaHCO₃胁迫的增加,地上部Na含量显著增加,而地上部K⁺含量则相反。在培养液中Na⁺浓度相同时,添加NaHCO₃导致地上部K⁺含量下降幅度更大,从而使地上部K⁺/Na⁺比值更低。与NaCl胁迫相比,在NaHCO₃胁迫下,叶片电解质渗漏率以及叶片丙二醛、脯氨酸和可溶性糖含量增加幅度更大。在NaCl和NaHCO₃胁迫下,叶片超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶(APX)和脱氢抗坏血酸还原酶(DHAR)活性显著诱导,而叶片过氧化物酶(POD)活性明显受到抑制。

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