Arteaga-Salas Jose Manuel, Harrison Andrew P, Upton Graham J G
University of Essex.
Stat Appl Genet Mol Biol. 2008;7(1):Article29. doi: 10.2202/1544-6115.1383. Epub 2008 Oct 17.
We address the problem of detection and correction of spatial flaws in oligonucleotide microarrays. We present two similar procedures, of which one is intended solely for use with replicates and the other has wider applicability. By constructing a set of replicates, with one realistically flawed, we are able to examine the extent to which our procedures are capable of repairing the flaw. We find that, for this purpose, our procedures are superior to the existing 'Harshlight' procedure.
我们探讨了寡核苷酸微阵列中空间缺陷的检测与校正问题。我们提出了两种类似的方法，其中一种仅适用于重复样本，另一种具有更广泛的适用性。通过构建一组重复样本，其中一个存在实际缺陷，我们能够检验我们的方法修复该缺陷的能力。我们发现，为此目的，我们的方法优于现有的“Harshlight”方法。
