Hoogduijn M J, Crop M J, Peeters A M A, Korevaar S S, Eijken M, Drabbels J J, Roelen D L, Maat A P M M, Balk A H M M, Weimar W, Baan C C
Deparmtent of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands.
Am J Transplant. 2009 Jan;9(1):222-30. doi: 10.1111/j.1600-6143.2008.02450.x. Epub 2008 Oct 31.
Mesenchymal stem cells (MSC) are characterized by their multilineage differentiation capacity and immunosuppressive properties. They are resident in virtually all tissues and we have recently characterized MSC from the human heart. Clinical heart transplantation offers a model to study the fate of transplanted human MSC. In this study, we isolated and expanded MSC from heart tissue taken before, and 1 week up to 6 years after heart transplantation. MSC from posttransplantation tissue were all of donor origin, demonstrating the longevity of endogenous MSC and suggesting an absence of immigration of recipient MSC into the heart. MSC isolated from transplanted tissue showed an immunophenotype that was characteristic for MSC and maintained cardiomyogenic and osteogenic differentiation capacity. They furthermore preserved their ability to inhibit the proliferative response of donor-stimulated recipient peripheral blood mononuclear cells. In conclusion, functional MSC of donor origin remain present in the heart for several years after transplantation.
间充质干细胞（MSC）的特征在于其多向分化能力和免疫抑制特性。它们几乎存在于所有组织中，我们最近对人心脏中的MSC进行了表征。临床心脏移植提供了一个研究移植的人MSC命运的模型。在本研究中，我们从心脏移植前以及移植后1周直至6年获取的心脏组织中分离并扩增了MSC。移植后组织中的MSC均来自供体，这证明了内源性MSC的长寿，并表明受体MSC没有迁移到心脏中。从移植组织中分离的MSC显示出MSC特有的免疫表型，并保持了心肌生成和成骨分化能力。它们还保留了抑制供体刺激的受体外周血单核细胞增殖反应的能力。总之，供体来源的功能性MSC在移植后数年仍存在于心脏中。
