下肢动脉闭塞患者血浆中对氧磷酶（PON1）的芳基酯酶和对氧磷酶活性受缺血影响。
Arylesterase and paraoxonase activity of paraoxonase (PON1) affected by ischemia in the plasma of patients with arterial occlusion of the lower limbs.
作者信息
Kasprzak Magdalena, Iskra Maria, Majewski Wacław, Wielkoszyński Tomasz
机构信息
Department of General Chemistry, Chemistry and Clinical Biochemistry, Poznań University of Medical Sciences, 6 Grunwaldzka Street, 60-780 Poznań, Poland.
出版信息
Clin Biochem. 2009 Jan;42(1-2):50-6. doi: 10.1016/j.clinbiochem.2008.09.117. Epub 2008 Oct 17.
OBJECTIVES
The aim of the study was to evaluate the effect of different degrees of chronic ischemia of the lower limbs on PON1 activity in plasma, in relation to different substrates.
DESIGN AND METHODS
The studied group consisted of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis. The paraoxonase and arylesterase activities of PON1 were measured according to the Karen Gan method.
RESULTS
PON1 arylesterase activity was affected by ischemia of the lower limbs depending on its degree. In the group with critical ischemia decreased PON1 activity was observed in comparison with that in the moderate ischemia group and the control group (50.05+/-21.40 U/mL and 82.59+/-29.27 U/mL, 85.30+/-35.05 U/mL, respectively).
CONCLUSIONS
The results revealed a reverse relationship between PON1 activity and the progress of atherosclerosis to the ischemic level. The present study demonstrates, for the first time, that the arylesterase activity of PON1 is affected by critical ischemia of the lower limbs.
目的
本研究旨在评估不同程度的下肢慢性缺血对血浆中对氧磷酶1（PON1）活性的影响，并探讨其与不同底物的关系。
设计与方法
研究组由因动脉粥样硬化导致下肢慢性动脉闭塞的患者组成。根据卡伦·甘（Karen Gan）方法测定PON1的对氧磷酶和芳基酯酶活性。
结果
PON1芳基酯酶活性受下肢缺血程度的影响。与中度缺血组和对照组相比，严重缺血组的PON1活性降低（分别为50.05±21.40 U/mL、82.59±29.27 U/mL和85.30±35.05 U/mL）。
结论
结果显示PON1活性与动脉粥样硬化进展至缺血水平之间呈负相关。本研究首次证明，PON1的芳基酯酶活性受下肢严重缺血的影响。
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