Sharma Shweta, Malik Anushree, Satya Santosh
Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi , India.
J Hazard Mater. 2009 May 30;164(2-3):1198-204. doi: 10.1016/j.jhazmat.2008.09.030. Epub 2008 Sep 17.
Response surface methodology (RSM) involving central composite design (CCD) was employed to optimize the concentrations of different media components for growth and Cr (VI) removal (100 mg l(-1) initial concentration) by Aspergillus lentulus AML05 at pH 6.5. The interaction between five variables i.e. glucose, K(2)HPO(4), MgSO(4), yeast extract, NH(4)NO(3) was studied and modelled. The statistical analysis of the results showed that in the range studied, yeast extract had a significant effect on Cr (VI) removal and production of fungal biomass. The optimum combination predicted via RSM was confirmed through experiment, whereby almost complete removal (99.8%) of Cr (VI) was obtained within 120 h. The validation of these results in terms of field applicability was also tested by treating industrial effluent supplemented with key media components.
采用包含中心复合设计（CCD）的响应面法（RSM），在pH 6.5条件下优化不同培养基成分的浓度，以实现慢生曲霉AML05的生长及对六价铬（初始浓度为100 mg l⁻¹）的去除。研究并模拟了五个变量即葡萄糖、磷酸氢二钾、硫酸镁、酵母提取物、硝酸铵之间的相互作用。结果的统计分析表明，在所研究的范围内，酵母提取物对六价铬的去除和真菌生物量的产生有显著影响。通过实验证实了经响应面法预测的最佳组合，在120小时内可实现几乎完全去除（99.8%）的六价铬。还通过处理添加了关键培养基成分的工业废水，测试了这些结果在实际应用方面的有效性。
