Ghosh Modhumita
Division of Plant Biotechnology, Institute of Forest Genetics and Tree Breeding, Forest Campus, R.S. Puram, Coimbatore-641 002, India.
Fitoterapia. 2009 Mar;80(2):91-5. doi: 10.1016/j.fitote.2008.10.004. Epub 2008 Oct 20.
A 30 KDa monomeric acidic lectin-like protein was purified from the leaves of an important medicinal herb, Withania somnifera (L.) Dunal (Solanaceae), by a series of gel filtration and affinity chromatography methods. The inhibitory concentration of the protein ranged from 7 microg to 11 microg against major phytopathogens under in vitro conditions. The peptide sequence showed similarity to concanavalin A like lectin from Canavalia ensiformis and caused distinct cell wall adhesion of the protein treated hyphae under SEM. Further, the antifungal activity of the protein was compared with standard lectins like concanavalin A, phytohemagglutinin and wheat germ agglutinin.
通过一系列凝胶过滤和亲和色谱方法，从一种重要的药用植物——印度人参（茄科）的叶子中纯化出一种30千道尔顿的单体酸性凝集素样蛋白。在体外条件下，该蛋白对主要植物病原体的抑制浓度范围为7微克至11微克。该肽序列与刀豆中的伴刀豆球蛋白A样凝集素相似，并且在扫描电子显微镜下，该蛋白处理过的菌丝表现出明显的细胞壁粘附现象。此外，还将该蛋白的抗真菌活性与伴刀豆球蛋白A、植物血凝素和麦胚凝集素等标准凝集素进行了比较。
