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具有高活性抗真菌、抗肿瘤和 HIV-1 逆转录酶抑制活性的高粱木聚糖酶抑制剂样蛋白。

A sorghum xylanase inhibitor-like protein with highly potent antifungal, antitumor and HIV-1 reverse transcriptase inhibitory activities.

机构信息

School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

出版信息

Food Chem. 2013 Dec 1;141(3):2916-22. doi: 10.1016/j.foodchem.2013.04.013. Epub 2013 Jun 5.

DOI:10.1016/j.foodchem.2013.04.013
PMID:23871041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7115760/
Abstract

A 25-kDa protein, with an N-terminal amino acid sequence homologous to that of xylanase inhibitor and designated as xylanase inbibitor-like protein (XILP) was purified from sorghum seeds. The isolation protocol consisted of affinity chromatography, ion exchange chromatography, and gel filtration. XILP inhibited mycelial growth in various phytopathogenic fungi. The antifungal activity was thermostable and pH-stable. XILP inhibited proliferation of various cancer cell lines but did not do so in human embryonic liver (WRL 68) cells. There was no mitogenic activity toward mouse splenocytes. XILP reduced the activity of HIV-1 reverse transcriptase with an IC50 of 11.1μM, but lacked inhibitory activity toward HIV-1 integrase and SARS coronavirus proteinase. In conclusion, sorghum XILP is thermostable and pH stable and exhibits potent antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities.

摘要

从高粱种子中纯化出一种 25kDa 的蛋白质，其 N 端氨基酸序列与木聚糖酶抑制剂同源，被命名为木聚糖酶抑制剂样蛋白（XILP）。该分离方案包括亲和层析、离子交换层析和凝胶过滤。XILP 抑制了各种植物病原真菌的菌丝生长。该抗真菌活性具有热稳定性和 pH 稳定性。XILP 抑制了各种癌细胞系的增殖，但对人胚肝（WRL 68）细胞没有抑制作用。对小鼠脾细胞没有有丝分裂活性。XILP 降低了 HIV-1 逆转录酶的活性，IC50 为 11.1μM，但对 HIV-1 整合酶和 SARS 冠状病毒蛋白酶没有抑制活性。总之，高粱 XILP 具有热稳定性和 pH 稳定性，表现出很强的抗真菌、抗增殖和 HIV-1 逆转录酶抑制活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/3204ef6b9994/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/4e9f7554525b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/a49ab02f346e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/53bc7f9770d4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/a89b3e7110e3/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/3204ef6b9994/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/4e9f7554525b/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/a49ab02f346e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/53bc7f9770d4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/a89b3e7110e3/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b02/7115760/3204ef6b9994/gr5.jpg

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