Marsillach Judit, Aragonès Gerard, Beltrán Raul, Caballeria Joan, Pedro-Botet Juan, Morcillo-Suárez Carlos, Navarro Arcadi, Joven Jorge, Camps Jordi
Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'Investigacions Sanitàries Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.
Clin Biochem. 2009 Jan;42(1-2):91-8. doi: 10.1016/j.clinbiochem.2008.09.120. Epub 2008 Oct 21.
We investigated the analytical performance of a new assay of the lactonase activity of paraoxonase-1 and its efficacy in the assessment of liver damage.
Serum lactonase activity was determined by the hydrolysis of 5-thiobutyl butyrolactone in 633 healthy individuals and 369 patients with chronic liver disease. Paraoxonase-1, 2, and 3 gene polymorphisms were analyzed by the MassArray method.
Linearity was up to 10 U/L. Detection limit was 0.12 U/L. Imprecision was < or = 17.7%. Lactonase values in our normal population were 5.99 (3.29-13.61) U/L. Lactonase activity showed a lower influence of genetic polymorphisms than the classical assay using paraoxon. Both measurements showed a similar efficiency in testing for liver dysfunction.
We report a reliable assay using a non-toxic substrate for the measurement of serum lactonase activity. The influence of genetic variability is low. The assay could be a useful addition to tests evaluating liver impairment.
我们研究了一种新的对氧磷酶-1内酯酶活性检测方法的分析性能及其在评估肝损伤方面的功效。
通过测定633名健康个体和369名慢性肝病患者血清中5-硫代丁基丁内酯的水解来确定血清内酯酶活性。采用MassArray方法分析对氧磷酶-1、2和3基因多态性。
线性范围高达10 U/L。检测限为0.12 U/L。不精密度≤17.7%。我们正常人群的内酯酶值为5.99（3.29 - 13.61）U/L。与使用对氧磷的经典检测方法相比，内酯酶活性受基因多态性的影响较小。两种检测方法在检测肝功能障碍方面显示出相似的效率。
我们报告了一种使用无毒底物测量血清内酯酶活性的可靠检测方法。遗传变异性的影响较低。该检测方法可能是评估肝损伤检测中的一个有用补充。
