显微注射埃及伊蚊胚胎以获得转基因蚊子。
Microinjection of A. aegypti embryos to obtain transgenic mosquitoes.
作者信息
Jasinskiene Nijole, Juhn Jennifer, James Anthony A
机构信息
Department of Molecular Biology and Biochemistry, University of California, Irvine, USA.
出版信息
J Vis Exp. 2007(5):219. doi: 10.3791/219. Epub 2007 Jul 4.
Abstract
In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.
摘要
在本视频中，尼约勒·亚辛斯基内展示了用于培育转基因埃及伊蚊的方法，埃及伊蚊是登革热的传播媒介。视频展示了正确准备显微注射针、干燥胚胎以及进行显微注射的技术。
相似文献
1
Microinjection of A. aegypti embryos to obtain transgenic mosquitoes.显微注射埃及伊蚊胚胎以获得转基因蚊子。
J Vis Exp. 2007(5):219. doi: 10.3791/219. Epub 2007 Jul 4.
2
Injection of An. stephensi embryos to generate malaria-resistant mosquitoes.注射斯氏按蚊胚胎以培育抗疟疾蚊子。
J Vis Exp. 2007(5):216. doi: 10.3791/216. Epub 2007 Jul 4.
3
Methods for TALEN Evaluation, Use, and Mutation Detection in the Mosquito Aedes aegypti.埃及伊蚊中TALEN评估、使用及突变检测的方法
Methods Mol Biol. 2016;1338:157-77. doi: 10.1007/978-1-4939-2932-0_13.
4
Building a better mosquito: identifying the genes enabling malaria and dengue fever resistance in A. gambiae and A. aegypti mosquitoes.培育更优良的蚊子：鉴定冈比亚按蚊和埃及伊蚊中赋予疟疾和登革热抗性的基因。
J Vis Exp. 2007(5):233. doi: 10.3791/233. Epub 2007 Jul 4.
5
Ethical, social, and cultural considerations for site selection for research with genetically modified mosquitoes.转基因蚊子研究地点选择的伦理、社会和文化考量
Am J Trop Med Hyg. 2008 Sep;79(3):312-8.
6
[Oral receptivity of Aedes aegypti formosus from Franceville (Gabon, central Africa) for type 2 dengue virus].[来自法国维尔（加蓬，中非）的福尔摩斯埃及伊蚊对2型登革病毒的经口易感性]
Bull Soc Pathol Exot. 1999 Dec;92(5):341-2.
7
8
Functional analysis of genes in Aedes aegypti embryos.埃及伊蚊胚胎中基因的功能分析。
Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5511. doi: 10.1101/pdb.prot5511.
9
Population dynamics of Aedes aegypti from a dengue hyperendemic urban setting in Colombia.来自哥伦比亚登革热高度流行城市环境的埃及伊蚊种群动态。
Am J Trop Med Hyg. 2004 Oct;71(4):506-13.
10
Low gene flow of Aedes aegypti between dengue-endemic and dengue-free areas in southeastern and southern Brazil.在巴西东南部和南部，登革热流行地区与无登革热地区之间埃及伊蚊的基因流动较低。
Am J Trop Med Hyg. 2007 Aug;77(2):303-9.
引用本文的文献
1
Harnessing the highly adaptable barnase-barstar system for genetic biocontrol of Aedes aegypti.利用高度适应性的芽孢杆菌RNA酶-芽孢杆菌RNA酶抑制剂系统对埃及伊蚊进行基因生物防治。
Commun Biol. 2025 Aug 4;8(1):1154. doi: 10.1038/s42003-025-08588-6.
2
Aedes aegypti VLG-1 challenges the assumed antiviral nature of Vago genes.埃及伊蚊VLG-1对Vago基因假定的抗病毒特性提出了挑战。
BMC Biol. 2025 Jul 28;23(1):223. doi: 10.1186/s12915-025-02325-5.
3
Cross-modal sensory compensation increases mosquito attraction to humans.跨模态感官补偿会增加蚊子对人类的吸引力。
Sci Adv. 2025 Jan 3;11(1):eadn5758. doi: 10.1126/sciadv.adn5758. Epub 2025 Jan 1.
4
The Development and Expansion of in vivo Germline Editing Technologies in Arthropods: Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) and Beyond.在节肢动物中体内种系编辑技术的发展和扩展：受体介导的卵母细胞转导 cargo（ReMOT Control）及其他方法。
Integr Comp Biol. 2023 Dec 29;63(6):1550-1563. doi: 10.1093/icb/icad123.
5
Fertility decline in female mosquitoes is regulated by the olfactory co-receptor.雌性蚊子的生育力下降受嗅觉共受体调控。
iScience. 2023 May 13;26(6):106883. doi: 10.1016/j.isci.2023.106883. eCollection 2023 Jun 16.
6
Aal-circRNA-407 regulates ovarian development of Aedes albopictus, a major arbovirus vector, via the miR-9a-5p/Foxl axis.Aal-circRNA-407 通过 miR-9a-5p/Foxl 轴调控白纹伊蚊（一种重要的虫媒病毒载体）的卵巢发育。
PLoS Pathog. 2023 May 5;19(5):e1011374. doi: 10.1371/journal.ppat.1011374. eCollection 2023 May.
7
A DBHS family member regulates male determination in the filariasis vector Armigeres subalbatus.一个 DBHS 家族成员调控丝虫病传播媒介致倦库蚊的雄性决定。
Nat Commun. 2023 Apr 21;14(1):2292. doi: 10.1038/s41467-023-37983-y.
8
Protocol to establish a genetically tractable synthetic symbiosis between Sodalis praecaptivus and grain weevils by insect egg microinjection.通过昆虫卵显微注射在俘获前 Sodalis 菌与谷象之间建立遗传易处理的合成共生关系的方案。
STAR Protoc. 2023 Mar 13;4(2):102156. doi: 10.1016/j.xpro.2023.102156.
9
The AalNix3&4 isoform is required and sufficient to convert Aedes albopictus females into males.AalNix3&4 同种型对于将白纹伊蚊雌性转变为雄性是必需且充分的。
PLoS Genet. 2022 Jun 23;18(6):e1010280. doi: 10.1371/journal.pgen.1010280. eCollection 2022 Jun.
10
Considerations for homology-based DNA repair in mosquitoes: Impact of sequence heterology and donor template source.考虑基于同源性的蚊子 DNA 修复：序列异质性和供体模板来源的影响。
PLoS Genet. 2022 Feb 18;18(2):e1010060. doi: 10.1371/journal.pgen.1010060. eCollection 2022 Feb.
Zotero 插件