冈比亚按蚊的饲养方案
Protocol for mosquito rearing (A. gambiae).
作者信息
Das Suchismita, Garver Lindsey, Dimopoulos George
机构信息
Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
出版信息
J Vis Exp. 2007(5):221. doi: 10.3791/221. Epub 2007 Jul 4.
Abstract
This protocol describes mosquito rearing in the insectary. The insectary rooms are maintained at 28 degrees C and approximately 80% humidity, with a 12 hr. day/night cycle. For this procedure, you'll need mosquito cages, 10% sterile sucrose solution, paper towels, beaker, whatman filter paper, glass feeders, human blood and serum, water bath, parafilm, distilled water, clean plastic trays, mosquito food (described below), mosquito net to cover the trays, vacuum, and a collection chamber to collect adults.
摘要
本方案描述了昆虫饲养室内蚊子的饲养方法。昆虫饲养室温度保持在28摄氏度，湿度约为80%，昼夜周期为12小时。进行此操作，你将需要蚊子饲养笼、10%无菌蔗糖溶液、纸巾、烧杯、沃特曼滤纸、玻璃喂食器、人血和血清、水浴锅、封口膜、蒸馏水、干净的塑料托盘、蚊子食物（如下所述）、覆盖托盘的蚊帐、真空吸尘器以及收集成虫的收集室。
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