Xi Zhiyong, Das Suchismita, Garver Lindsey, Dimopoulos George
Malaria Research Institute, Bloomberg School of Public Health, John Hopkins University, USA.
J Vis Exp. 2007(5):222. doi: 10.3791/222. Epub 2007 Jul 4.
Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
一旦某个基因被确定可能对疟疾具有难治性，就必须评估其在防止疟原虫在蚊子体内感染方面的作用。本方案说明了如何测定蚊子的疟原虫感染程度。展示了制备配子体培养物、用人类血液膜饲蚊子以及测定蚊子中肠病毒滴度的技术。
