Xu Xiang-Jiao, Chen Chun-Qiao, Wang Zhuo-Yuan, Zhang Yi, Hou An-Xin, Li Chao-Hong, Liu Yi
State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, China.
Biol Trace Elem Res. 2009 Mar;127(3):269-77. doi: 10.1007/s12011-008-8243-3. Epub 2008 Nov 1.
The antibacterial effect of Zn(II), tetraphenyl porphyrin (TPP), propdioxyl bridged tetraphenyl bisporphyrin 1, and its metallobisporphyrin complexes (ZnMnbisporphyrin 2 and ZnZnbisporphyrin 3) towards Staphylococcus aureus growth was investigated by microcalorimetry at 37 degrees C. Differences in their capacities to inhibit the growth metabolism of S. aureus were observed. By analyzing the power-time curves, crucial parameters such as the rate constant of bacterial growth (k), inhibitory ratio (I), and generation time (t (G)) were determined. The growth rate constant (k) of S. aureus (in the log phase) in the presence of the drugs decreased linearly with increasing concentrations of the complexes. The sequence of the antibacterial activities of these compounds tested was 3 > 2 > 1 > Zn(II) > TPP. ZnZnbisporphyrin 3 is proposed to benefit from the synergetic effects of Zn(II) and 1.
在37℃下，通过微量量热法研究了锌（II）、四苯基卟啉（TPP）、丙二氧基桥联四苯基双卟啉1及其金属双卟啉配合物（锌锰双卟啉2和锌锌双卟啉3）对金黄色葡萄球菌生长的抗菌作用。观察到它们抑制金黄色葡萄球菌生长代谢能力的差异。通过分析功率-时间曲线，确定了诸如细菌生长速率常数（k）、抑制率（I）和代时（t（G））等关键参数。在药物存在下，金黄色葡萄球菌（对数期）的生长速率常数（k）随配合物浓度的增加呈线性下降。所测试的这些化合物的抗菌活性顺序为3＞2＞1＞锌（II）＞TPP。锌锌双卟啉3被认为受益于锌（II）和1的协同效应。
