Inoue Naokazu, Okabe Masaru
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
Methods Mol Biol. 2008;475:335-45. doi: 10.1007/978-1-59745-250-2_19.
As representatives of the 60 trillion cells that make a human body, a sperm and an egg meet, recognize each other, and fuse to create a new generation. Thus, gamete fusion is an extremely important process that must transpire without error to launch life activity. This may drive the fusion mechanism to evolve into an unfailing and steady process. At the same time, fusion must be restricted to occur only between two gametes of the same species. However, the molecular bases of the fusion event in fertilization have not yet been clarified. In this chapter, we describe the methods to evaluate fusion by staining the swollen sperm nuclei after fertilization.
作为构成人体的60万亿个细胞的代表，精子与卵子相遇、相互识别并融合，从而孕育出新一代。因此，配子融合是一个极其重要的过程，必须准确无误地发生才能启动生命活动。这可能促使融合机制演变成一个可靠且稳定的过程。同时，融合必须受到限制，只能发生在同一物种的两个配子之间。然而，受精过程中融合事件的分子基础尚未阐明。在本章中，我们描述了通过对受精后肿胀的精核进行染色来评估融合的方法。
