Hunter Chris, Evans Jennifer, Valencik Maria L
University of Utah, Nora Eccles Harrison Cardiovascular Research and Training Institute, Salt Lake City, Utah, USA.
Cell Commun Adhes. 2008 Sep;15(3):247-60. doi: 10.1080/15419060802198660.
Yeast two-hybrid analysis (Fields and Song, 1989, Nature, 340:245-246) was used to screen a human heart library to isolate proteins interacting with the adult muscle-specific beta1D integrin but not with beta1A integrin. In addition to previously identified interactions (RACK 1(Liliental and Chang, 1998, Journal of Biological Chemistry, 273:2379-2383) and alpha-actinin (Otey et al., 1990, Journal of Cell Biology, 111:721-729), the authors isolated several novel candidates. These include subunit 3 (CSN3/Sgn3) of the COP9 signalosome complex, cyclins D1, D2, and D3, RanBPM, and a recently identified protein COG8/DOR1. These protein interactions were specific for beta1D integrin, as no binding to beta1A integrin cytoplasmic domain was measurable by two-hybrid analysis. This paper presents the initial characterization of the interaction of CSN3 with beta1D integrin, the localization of CSN3 and the other COP9 signalosome subunits in embryonic and adult cardiac myocytes and their response to muscle cell differentiation.
酵母双杂交分析（Fields和Song，1989年，《自然》，340:245 - 246）用于筛选人心脏文库，以分离与成年肌肉特异性β1D整合素相互作用但不与β1A整合素相互作用的蛋白质。除了先前已确定的相互作用（RACK 1（Liliental和Chang，1998年，《生物化学杂志》，273:2379 - 2383）和α - 辅肌动蛋白（Otey等人，1990年，《细胞生物学杂志》，111:721 - 729））外，作者还分离出了几个新的候选蛋白。这些包括COP9信号体复合物的亚基3（CSN3/Sgn3）、细胞周期蛋白D1、D2和D3、RanBPM以及最近鉴定的蛋白COG8/DOR1。这些蛋白质相互作用对β1D整合素具有特异性，因为通过双杂交分析无法检测到与β1A整合素胞质结构域的结合。本文介绍了CSN3与β1D整合素相互作用的初步特征、CSN3和其他COP9信号体亚基在胚胎和成年心肌细胞中的定位及其对肌肉细胞分化的反应。
