Klemke Markus, Meyer Anke, Nezhad Maliheh Hashemi, Bartnitzke Sabine, Drieschner Norbert, Frantzen Christiane, Schmidt Ernst Heinrich, Belge Gazanfer, Bullerdiek Jörn
Center for Human Genetics, University of Bremen, 28359 Bremen, Germany.
Genes Chromosomes Cancer. 2009 Feb;48(2):171-8. doi: 10.1002/gcc.20627.
An overexpression of HMGA2 is supposed to be a key event in the genesis of leiomyoma with chromosomal rearrangements affecting the region 12q14-15 targeting the HMGA2 gene, but gene expression data regarding differences between uterine leiomyomas with and those without 12q14-15 aberrations are insufficient. To address the question whether HMGA2 is only upregulated in the 12q14-15 subgroup, the expression of HMGA2 was analyzed in a comprehensive set of leiomyomas (n = 180) including tumors with 12q14-15 chromosomal aberrations (n = 13) and matching myometrial tissues (n = 51) by quantitative RT-PCR. The highest expression levels for HMGA2 were observed in tumors with rearrangements affecting the region 12q14-15, but although HMGA2 is expressed at lower levels in leiomyomas without such aberrations, the comparison between the expression in myomas and matching myometrial tissues indicates a general upregulation of HMGA2 regardless of the presence or absence of such chromosomal abnormalities. The significant (P < 0.05) overexpression of HMGA2 also in the group of fibroids without chromosomal aberrations of the 12q14-15 region suggests a general role of HMGA2 in the development of the disease.
HMGA2的过表达被认为是平滑肌瘤发生过程中的关键事件，其中染色体重排影响靶向HMGA2基因的12q14 - 15区域，但关于有和没有12q14 - 15畸变的子宫平滑肌瘤之间差异的基因表达数据不足。为了解决HMGA2是否仅在12q14 - 15亚组中上调的问题，通过定量RT - PCR分析了一组全面的平滑肌瘤（n = 180）中HMGA2的表达，包括有12q14 - 15染色体重排的肿瘤（n = 13）和匹配的肌层组织（n = 51）。在影响12q14 - 15区域的重排肿瘤中观察到HMGA2的最高表达水平，但是尽管HMGA2在没有此类畸变的平滑肌瘤中表达水平较低，但肌瘤与匹配的肌层组织之间的表达比较表明，无论是否存在此类染色体异常，HMGA2总体上均上调。在没有12q14 - 15区域染色体畸变的肌瘤组中HMGA2也有显著（P < 0.05）过表达，这表明HMGA2在该疾病发展中具有普遍作用。
