Jischa Susanne, Walter Ingrid, Nowotny Norbert, Palm Franziska, Budik Sven, Kolodziejek Jolanta, Aurich Christine
Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Sciences, 1210 Vienna, Austria.
Am J Vet Res. 2008 Nov;69(11):1525-34. doi: 10.2460/ajvr.69.11.1525.
To determine endometrial regeneration in postpartum mares by analysis of histologic features, apoptosis and cell proliferation markers, lectin binding, cytokines, and progesterone and estrogen receptors in endometrial biopsy specimens.
9 postpartum mares.
Mares were examined on postpartum days 1, 9, and 16, and uterine biopsy specimens were obtained for histologic examination. Lectin binding was analyzed histochemically, and expressions of Ki-67 antigen (proliferation marker), lysozyme, and caspase 3 (apoptosis marker) were studied immunohistochemically. Gene expressions for cytokines (interleukin-1beta, -6 and -8 and tumor necrosis factor-alpha), cyclooxygenase 2, prostaglandin-E-synthase, and estrogen and progesterone receptors were determined by use of quantitative real-time PCR assay.
On day 1, neutrophils predominated but by day 9 had largely been replaced by lymphocytes and macrophages. High numbers of cells with staining for caspase 3 were found on day 1, but numbers decreased by day 9. In contrast, the number of cells with staining for Kiel 67 antigen increased between days 1 and 9. Lectin binding to the endometrium changed over time. Relative mRNA expressions for cytokines and prostaglandin-E-synthase did not differ among days. Expressions of progesterone and estrogen receptors were minimal on day 1 and increased by day 9.
Early postpartum endometrial cells underwent apoptosis, but during the second week, postpartum proliferation of cells predominated. Lectin binding reflected changes in endometrial glycocalyx patterns. Increased expression of estrogen receptors allowed the endometrium to respond to estrogen during foal heat, and in subsequent diestrus, the endometrium was able to respond to progesterone.
通过分析子宫内膜活检标本的组织学特征、细胞凋亡和细胞增殖标志物、凝集素结合、细胞因子以及孕酮和雌激素受体，确定产后母马子宫内膜的再生情况。
9匹产后母马。
在产后第1天、第9天和第16天对母马进行检查，并获取子宫活检标本进行组织学检查。采用组织化学方法分析凝集素结合情况，采用免疫组织化学方法研究Ki-67抗原（增殖标志物）、溶菌酶和半胱天冬酶3（凋亡标志物）的表达。通过定量实时PCR测定细胞因子（白细胞介素-1β、-6和-8以及肿瘤坏死因子-α）、环氧化酶2、前列腺素-E合成酶以及雌激素和孕酮受体的基因表达。
第1天中性粒细胞占主导，但到第9天大部分已被淋巴细胞和巨噬细胞取代。第1天发现大量半胱天冬酶3染色阳性的细胞，但到第9天数量减少。相比之下，第1天到第9天Ki-67抗原染色阳性的细胞数量增加。凝集素与子宫内膜的结合随时间变化。细胞因子和前列腺素-E合成酶的相对mRNA表达在各天之间无差异。孕酮和雌激素受体的表达在第1天最低，到第9天增加。
产后早期子宫内膜细胞发生凋亡，但在第二周，产后细胞增殖占主导。凝集素结合反映了子宫内膜糖萼模式的变化。雌激素受体表达增加使子宫内膜在产后第一次发情期能够对雌激素作出反应，在随后的间情期，子宫内膜能够对孕酮作出反应。
