Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Olsztyn, Poland.
Biol Reprod. 2013 Oct 4;89(4):79. doi: 10.1095/biolreprod.113.109447. Print 2013 Oct.
The aim of the study was to characterize endometrial mRNA transcription, immunolocalization, and protein expression of interleukin (IL) 1alpha, IL1beta, IL6, and IL1RI, IL1RII, and IL6Ralpha/beta in the course of endometrosis during the estrous cycle. Additionally, the influence of IL1alpha, IL1beta, and IL6 on prostaglandin (PG) secretion and PG synthase mRNA transcription in endometrial tissue during endometrosis was investigated. The endometrial samples were obtained at the early (n = 12), mid- (n = 12), and late (n = 12) luteal phases and at the follicular (n = 12) phase of the estrous cycle. Within each of these phases, there were four samples within each category I, II, and III of endometrium, according to the Kenney classification. In experiment 1, transcription of IL1alpha, IL1beta, IL6, and their receptor's (IL1RI, IL1RII, and IL6Ralpha/beta) mRNAs and their immunolocalization and protein expression were determined using real-time PCR and immunohistochemistry, respectively. In Experiment 2, endometrial samples (n = 5 samples within categories I, II, and III) were obtained for tissue culture in the midluteal phase of the estrous cycle. The endometrial tissues were stimulated with IL1alpha (10 ng/ml), IL1beta (10 ng/ml), IL6 (10 ng/ml), and oxytocin (positive control; 10⁻⁷ M) for 24 h. The PG concentration was determined using ELISA. In addition, transcription of PTGS-2, PGES, and PGFS mRNAs was determined using real-time PCR. ILs were found to regulate PG secretion via modulation of PG synthases in equine endometrium. The alterations in IL and the expression of their receptors, and in endometrial secretory functions, were observed during the course of endometrosis, and suggest serious changes in the endometrial microenvironment. The described disturbances may be closely related to impaired endometrial processes responsible for the subfertility or the infertility in endometrosis.
本研究旨在描述子宫内膜 mRNA 转录、白细胞介素（IL）1α、IL1β、IL6 及其受体（IL1RI、IL1RII 和 IL6Rα/β）在发情周期中子宫内膜异位症过程中的免疫定位和蛋白表达。此外，还研究了 IL1α、IL1β 和 IL6 对子宫内膜异位症中子宫内膜组织前列腺素（PG）分泌和 PG 合酶 mRNA 转录的影响。在发情周期的早（n = 12）、中（n = 12）和晚（n = 12）黄体期以及卵泡期（n = 12）获得子宫内膜样本。在这些阶段的每个阶段，根据 Kenney 分类，每个类别 I、II 和 III 的子宫内膜中都有四个样本。在实验 1 中，使用实时 PCR 和免疫组织化学分别测定 IL1α、IL1β、IL6 及其受体（IL1RI、IL1RII 和 IL6Rα/β）mRNA 的转录及其免疫定位和蛋白表达。在实验 2 中，在发情周期的中黄体期获得子宫内膜样本（n = 5 个类别 I、II 和 III 中的样本）进行组织培养。用白细胞介素 1α（10ng/ml）、白细胞介素 1β（10ng/ml）、白细胞介素 6（10ng/ml）和催产素（阳性对照；10⁻⁷ M）刺激子宫内膜组织 24 小时。使用 ELISA 测定 PG 浓度。此外，使用实时 PCR 测定 PGES、PGFS 和 PTGS-2 mRNA 的转录。在马子宫内膜中，IL 通过调节 PG 合酶来调节 PG 分泌。在子宫内膜异位症过程中观察到 IL 和其受体的表达以及子宫内膜分泌功能的改变，表明子宫内膜微环境发生严重变化。所描述的紊乱可能与子宫内膜过程受损密切相关，这些过程负责子宫内膜异位症中的不孕或不育。
